Fries Brian D, Hummon Amanda B
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA; email:
Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2025 May;18(1):173-191. doi: 10.1146/annurev-anchem-061424-090547. Epub 2025 Jan 14.
The use of cell culture techniques to model human disease is an indispensable tool that has helped improve the health and well-being of the world. Monolayer cultures have most often been used for biomedical research, although not accurately recapitulating an in vivo human tumor. Tumor spheroids are a form of three-dimensional cell culture that better mimics an avascularized human tumor through their cell-cell contacts in all directions, development of various chemical gradients, and distinct populations of cells found within the spheroid. In this review, we highlight how mass spectrometry has propelled the utility of the spheroid model to understand cancer biology. We discuss how mass spectrometry imaging can be utilized to determine the penetration efficiency of various chemotherapeutics, how proteomics can be used to understand the biology in the various layers of a spheroid, and how metabolomics and lipidomics are used to elucidate how various spheroids behave toward chemotherapeutics.
利用细胞培养技术来模拟人类疾病是一种不可或缺的工具，它有助于改善全球人类的健康和福祉。单层培养最常用于生物医学研究，尽管它无法精确重现体内人类肿瘤的情况。肿瘤球体是三维细胞培养的一种形式，通过其在各个方向上的细胞间接触、各种化学梯度的形成以及球体内部不同细胞群体的存在，能更好地模拟无血管化的人类肿瘤。在本综述中，我们强调了质谱技术如何推动了球体模型在理解癌症生物学方面的应用。我们讨论了如何利用质谱成像来确定各种化疗药物的渗透效率，如何利用蛋白质组学来理解球体各层的生物学特性，以及如何利用代谢组学和脂质组学来阐明各种球体对化疗药物的反应。
