Shaykevich Sarah F, Little Justin P, Qian Yong, Paquet Marie-Eve, Campbell Robert E, Razansky Daniel, Shoham Shy
NYU Langone Health, Tech4Health and Neuroscience Institutes, and Department of Ophthalmology, New York City, USA.
Massachusetts Institute of Technology, McGovern Institute for Brain Research, Cambridge, MA, USA.
Photoacoustics. 2024 Nov 28;41:100671. doi: 10.1016/j.pacs.2024.100671. eCollection 2025 Feb.
Measuring whole-brain distributed functional activity is an important unmet need in neuroscience, requiring high temporal resolution and cellular specificity across large volumes. Functional optoacoustic neuro-tomography (FONT) with genetically encoded calcium ion indicators is a promising approach towards this goal. However, it has not yet been applied in the near-infrared (NIR) range that provides deep penetration and low vascular background optimal for neuroimaging. Here, we study the noninvasive multimodal fluorescence and optoacoustic imaging performance of state-of-the-art NIR calcium ion indicator NIR-GECO2G in the mouse brain. We observe robust signals with widefield fluorescence, and for the first time, with FONT. We also show that in both modalities, the NIR-GECO2G signal improves more than twofold in the biliverdin-enriched mouse line compared to wild type. Our findings demonstrate the potential of multimodal fluorescence and optoacoustic NIR imaging, opening new possibilities for whole-brain real-time functional imaging in rodents.
测量全脑分布式功能活动是神经科学中一项尚未满足的重要需求，需要高时间分辨率和跨越大量体积的细胞特异性。使用基因编码钙离子指示剂的功能光声神经断层扫描（FONT）是实现这一目标的一种很有前景的方法。然而，它尚未应用于近红外（NIR）范围，该范围可为神经成像提供深度穿透和低血管背景的最佳条件。在这里，我们研究了最先进的近红外钙离子指示剂NIR-GECO2G在小鼠大脑中的无创多模态荧光和光声成像性能。我们通过宽场荧光首次观察到了来自FONT的强大信号。我们还表明，在这两种模式下，与野生型相比，在富含胆绿素的小鼠品系中，NIR-GECO2G信号提高了两倍多。我们的研究结果证明了多模态荧光和近红外光声成像的潜力，为啮齿动物的全脑实时功能成像开辟了新的可能性。
