Deán-Ben X Luís, Gottschalk Sven, Sela Gali, Shoham Shy, Razansky Daniel
Opt Lett. 2017 Mar 1;42(5):959-962. doi: 10.1364/OL.42.000959.
Genetically-encoded calcium indicators (GECIs) have revolutionized neuroimaging by enabling mapping of the activity of entire neuronal populations in vivo. Visualization of these powerful activity sensors has to date been limited to depth-restricted microscopic studies due to intense light scattering in the brain. We demonstrate, for the first time, in vivo real-time volumetric optoacoustic monitoring of calcium transients in adult transgenic zebrafish expressing the GCaMP5G calcium indicator. Fast changes in optoacoustic traces associated with GCaMP5G activity were detectable in the presence of other strongly absorbing endogenous chromophores, such as hemoglobin. The new functional optoacoustic neuroimaging method can visualize neural activity at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques.
基因编码钙指示剂（GECIs）通过实现对体内整个神经元群体活动的映射，给神经成像带来了革命性变化。由于大脑中强烈的光散射，迄今为止，这些强大的活动传感器的可视化仅限于深度受限的微观研究。我们首次在表达GCaMP5G钙指示剂的成年转基因斑马鱼中，对钙瞬变进行了体内实时体积光声监测。在存在其他强吸收性内源性发色团（如血红蛋白）的情况下，与GCaMP5G活性相关的光声信号的快速变化是可检测到的。这种新的功能性光声神经成像方法能够在现有神经成像技术无法覆盖的穿透深度和时空分辨率尺度上可视化神经活动。
