Deán-Ben X Luís, Sela Gali, Lauri Antonella, Kneipp Moritz, Ntziachristos Vasilis, Westmeyer Gil G, Shoham Shy, Razansky Daniel
Institute for Biological and Medical Imaging (IBMI), Helmholtz Center Munich, Neuherberg, Germany.
Institute of Developmental Genetics, Helmholtz Center Munich, Neuherberg, Germany.
Light Sci Appl. 2016 Dec 2;5(12):e16201. doi: 10.1038/lsa.2016.201. eCollection 2016 Dec.
Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience. We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains. It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm with respective spatial resolution of 35-200 μm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering brains. The newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics; in combination with optoacoustics' well-established capacity for resolving vascular hemodynamics, it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.
对分布于整个大脑的大型神经群体的时空活动进行无创观察是神经科学的一个长期目标。我们开发了一种体积多光谱光声断层扫描平台，用于对散射脑深部的神经激活进行成像。它可以在50至1000毫米的可扩展视野范围内每秒记录100个体积帧，各自的空间分辨率为35 - 200微米。在固定和自由游动的幼虫以及表达基因编码钙指示剂GCaMP5G的成年斑马鱼大脑中进行的实验首次证明了利用光声直接追踪神经动力学的基本能力，同时克服了散射脑光学成像长期存在的穿透障碍。因此，新开发的平台为快速钙动力学的功能性全脑观察提供了前所未有的能力；结合光声在解析血管血流动力学方面已确立的能力，它可以为健康和疾病状态下神经活动和神经血管耦合的研究开辟新的前景。
