Su Yajuan, Liu Tiantian, Zhao Minjun, Wu Dandan, Wang Yuehua, Wu Xubo
School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Department of Orthopedic Rehabilitation, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, 200137, China.
Heliyon. 2024 Dec 12;11(1):e41087. doi: 10.1016/j.heliyon.2024.e41087. eCollection 2025 Jan 15.
Schwann cells, as crucial regenerative cells, possess the ability to facilitate axon growth following peripheral nerve injury. However, the regeneration efficiency dominated by Schwann cells is impaired by factors such as the severity of peripheral nervous injury, aging, and metabolic disease. Cause the limitations of clinical treatments, it is necessary to urgently search for new substances that could reinforce the functionality of Schwann cells and promote nerve regeneration. We represented the first evidence that isoviolanthin possesses the capability to enhance Schwann cell proliferation and migration. Then, transcriptome sequencing was employed to examine the Differential Expressed Genes (DEGs), resulting in the identification of 193 DEGs. Following this, the expression levels of the top 5 up-regulated genes were confirmed through RT-qPCR, with Fhl3 demonstrating the most significant up-regulation. Schwann cells were transduced with virus particles made in HEK-293T/17 cells by transfection with lentivirus packaging plasmids containing Fhl3. A notable enhancement in Schwann cell proliferation and migration was observed following transduction. Furthermore, the Fhl3-up group exhibited a significant upregulation of Vimentin expression compared to the control group. These results suggested that isoviolanthin plays a positive role in enhancing Schwann cells' activity via increasing Fhl3 expression, and the mechanism may be related to the EMT(epithelial-mesenchymal transition)-like process.
雪旺细胞作为关键的再生细胞，具有促进周围神经损伤后轴突生长的能力。然而，由雪旺细胞主导的再生效率会受到周围神经损伤的严重程度、衰老和代谢疾病等因素的影响。由于临床治疗的局限性，迫切需要寻找能够增强雪旺细胞功能并促进神经再生的新物质。我们首次证明异紫堇黄质具有增强雪旺细胞增殖和迁移的能力。然后，采用转录组测序来检测差异表达基因（DEGs），共鉴定出193个DEGs。随后，通过RT-qPCR证实了前5个上调基因的表达水平，其中Fhl3的上调最为显著。通过用含有Fhl3的慢病毒包装质粒转染HEK-293T/17细胞制备病毒颗粒，转导雪旺细胞。转导后观察到雪旺细胞增殖和迁移显著增强。此外，与对照组相比，Fhl3上调组波形蛋白表达显著上调。这些结果表明，异紫堇黄质通过增加Fhl3表达对增强雪旺细胞活性起积极作用，其机制可能与类上皮-间质转化（EMT）过程有关。
