鸭和人类乙型肝炎病毒基因组的比较序列分析。

Comparative sequence analysis of duck and human hepatitis B virus genomes.

作者信息

Sprengel R, Kuhn C, Will H, Schaller H

出版信息

J Med Virol. 1985 Apr;15(4):323-33. doi: 10.1002/jmv.1890150402.

Abstract

We have cloned and sequenced an infectious, functionally active genome of a duck hepatitis B virus (DHBV). It is 3,021 base pairs (bp) in length and shows little DNA sequence homology to the genome of human hepatitis B virus (HBV). However, the amino acid sequences of predicted viral gene products are similar between DHBV and HBV, and the genome organization present in DHBV reflects that of HBV. As in the mammalian virus the long minus strand of the DHBV genome encodes three long overlapping reading frames designated as P, S, and C. The fourth open reading frame, termed X, is absent in DHBV. A comparison with a sequence of a second DHBV isolate [Mandart et al, Journal of Virology 49:782-792, 1984] revealed a nucleotide sequence variation of 5.6% and confirmed the presented overall gene organization of DHBV.

摘要

我们已经克隆并测序了鸭乙型肝炎病毒（DHBV）具有感染性、功能活跃的基因组。它长度为3021个碱基对（bp），与人类乙型肝炎病毒（HBV）的基因组几乎没有DNA序列同源性。然而，DHBV和HBV之间预测的病毒基因产物的氨基酸序列相似，并且DHBV中的基因组结构反映了HBV的结构。与哺乳动物病毒一样，DHBV基因组的长负链编码三个长的重叠阅读框，分别命名为P、S和C。DHBV中不存在第四个开放阅读框，称为X。与第二个DHBV分离株的序列[曼达特等人，《病毒学杂志》49:782 - 792，1984]进行比较，发现核苷酸序列变异为5.6%，并证实了所呈现的DHBV的整体基因结构。