Uchida M, Esumi M, Shikata T
First Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Virology. 1989 Dec;173(2):600-6. doi: 10.1016/0042-6822(89)90571-0.
The genomes of duck hepatitis B virus (DHBV) from a brown duck (S5) and a white duck (S31) kept independently in Shanghai, China, were cloned and the complete nucleotide sequence of each virion DNA (DHBV-S5 and DHBV-S31) was determined. DHBV-S5 and DHBV-S31 were both 3027 bp in length and 6 bp longer than the other two DHBVs analyzed previously, DHBV16 and DHBV3. The genomes of DHBV-S5 and DHBV-S31 encoded three long overlapping open reading frames designated as P, S, and C. A possible new open reading frame was found in a complementary strand of each viral genome, as 336 bp for DHBV-S5 and 306 bp for DHBV-S31, respectively. A pair of 3-bp insertions were found in the overlapping region of pre-S2 and P and so two amino acids were inserted in this region in DHBV-S5 and DHBV-S31. The nucleotide sequence variation between DHBV-S5 and DHBV-S31 (4.9%) was similar to that between DHBV16 and DHBV3 (5.6%), and less than the variations between either of these Shanghai clones and DHBV16 or DHBV3 (9.5-10.4%). The amino acid sequence was also conserved in the two Shanghai clones but showed group difference from DHBV16 or DHBV3. Thus these two independent Shanghai clones of DHBV showed geographical characteristics of genomic structure.
对在中国上海独立饲养的一只棕鸭（S5）和一只白鸭（S31）体内的鸭乙型肝炎病毒（DHBV）基因组进行了克隆，并测定了每个病毒粒子DNA（DHBV-S5和DHBV-S31）的完整核苷酸序列。DHBV-S5和DHBV-S31的长度均为3027 bp，比之前分析的另外两种DHBV（DHBV16和DHBV3）长6 bp。DHBV-S5和DHBV-S31的基因组编码三个长的重叠开放阅读框，分别命名为P、S和C。在每个病毒基因组的互补链中发现了一个可能的新开放阅读框，DHBV-S5为336 bp，DHBV-S31为306 bp。在pre-S2和P的重叠区域发现了一对3 bp的插入，因此在DHBV-S5和DHBV-S31的该区域插入了两个氨基酸。DHBV-S5和DHBV-S31之间的核苷酸序列变异（4.9%）与DHBV16和DHBV3之间的变异（5.6%）相似，且小于这两个上海克隆株与DHBV16或DHBV3之间的变异（9.5 - 10.4%）。这两个上海克隆株的氨基酸序列也保守，但与DHBV16或DHBV3存在群体差异。因此，这两个独立的上海DHBV克隆株显示出基因组结构的地理特征。
