Denti Vanna, Serrao Simone, Bossi Eleonora, Paglia Giuseppe
Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro, Italy.
Methods Mol Biol. 2025;2891:221-237. doi: 10.1007/978-1-0716-4334-1_12.
Trapped ion mobility spectrometry (TIMS) using parallel accumulation serial fragmentation (PASEF) is an advanced analytical technique that offers several advantages in mass spectrometry (MS)-based lipidomics. TIMS provides an additional dimension of separation to mass spectrometry and accurate collision cross-section (CCS) measurements for ions, aiding in the structural characterization of molecules. This is especially valuable in lipidomics for identifying and distinguishing isomeric or structurally similar compounds. On the other hand, PASEF technology allows for fast and efficient data acquisition by accumulating ions in parallel and then serially fragmenting them. This accelerates the analysis process and improves throughput, making it suitable for high-throughput applications. Moreover, the combination of TIMS and PASEF reduces co-elution and ion coalescence issues, leading to cleaner and more interpretable mass spectra. This results in higher data quality and more confident identifications. In this chapter, a data-dependent TIMS-PASEF workflow for lipidomics analysis is presented.
采用平行累积串联碎裂（PASEF）的捕集离子淌度质谱法（TIMS）是一种先进的分析技术，在基于质谱（MS）的脂质组学中具有诸多优势。TIMS为质谱提供了额外的分离维度，并能对离子进行精确的碰撞截面（CCS）测量，有助于分子的结构表征。这在脂质组学中对于鉴定和区分同分异构体或结构相似的化合物尤为重要。另一方面，PASEF技术通过并行累积离子然后对其进行串联碎裂，实现快速高效的数据采集。这加速了分析过程并提高了通量，使其适用于高通量应用。此外，TIMS和PASEF的结合减少了共洗脱和离子合并问题，从而得到更纯净、更易解释的质谱图。这带来了更高的数据质量和更可靠的鉴定结果。在本章中，将介绍一种用于脂质组学分析的数据依赖型TIMS-PASEF工作流程。
