Dolu Hüsna, Killi Dilek, Bas Serpil, Bilecen Deniz Sezlev, Seymen Musa
National Research Council of Italy-Institute of Sustainable Plant Protection (CNR-IPSP), Firenze, Italy.
Department of Plant Production and Technologies, Konya Food and Agriculture University, Konya, Turkey.
Photosynth Res. 2025 Jan 16;163(1):7. doi: 10.1007/s11120-024-01128-z.
Seed priming and plant growth-promoting bacteria (PGPB) may alleviate salt stress effects. We exposed a salt-sensitive variety of melon to salinity following seed priming with NaCl and inoculation with Bacillus. Given the sensitivity of photosystem II (PSII) to salt stress, we utilized dark- and light-adapted chlorophyll fluorescence alongside analysis of leaf stomatal conductance of water vapour (G). Priming increased total seed germination by 15.5% under salt-stress. NaCl priming with Bacillus inoculation (PB) increased total leaf area (LA) by 45% under control and 15% under stress. Under the control condition, priming (P) reduced membrane permeability (RMP) by 36% and PB by 55%, while under stress Bacillus (BS) reduced RMP by 10%. Although Bacillus inoculation (B) and priming (P) treatments did not show significant effects on some PSII efficiency parameters (F/F, ABS/RC, PI, F), the BS treatment induced a significantly higher quantum efficiency of PSII (ΦPSII) and increased G by 159% in the final week of the experiment. The BS treatment reduced electron transport rate per reaction center (ET/RC) by 10% in comparison to the salt treatment, which showed less reaction centre damage. Bacillus inoculation and seed priming treatment under the stressed condition (PBS) induced an increase in electron transport rate of 40%. Salt stress started to show significant effects on PSII after 12 days, and adversely impacted all morphological and photosynthetic parameters after 22 days. Salt priming and PGPB mitigated the negative impacts of salt stress and may serve as effective tools in future-proofing saline agriculture.
种子引发和植物促生细菌（PGPB）可能会减轻盐胁迫的影响。我们在用NaCl对种子进行引发处理并接种芽孢杆菌后，将一个盐敏感型甜瓜品种暴露于盐胁迫环境中。鉴于光系统II（PSII）对盐胁迫敏感，我们利用了暗适应和光适应叶绿素荧光技术，同时分析了叶片水汽气孔导度（G）。在盐胁迫下，引发处理使种子总发芽率提高了15.5%。在对照条件下，NaCl引发并接种芽孢杆菌（PB）使总叶面积（LA）增加了45%，在胁迫条件下增加了15%。在对照条件下，引发处理（P）使膜通透性（RMP）降低了36%，PB处理使其降低了55%，而在胁迫条件下，芽孢杆菌处理（BS）使RMP降低了10%。尽管接种芽孢杆菌（B）和引发处理（P）对一些PSII效率参数（F/F、ABS/RC、PI、F）没有显著影响，但在实验的最后一周，BS处理诱导出显著更高的PSII量子效率（ΦPSII），并使G增加了159%。与盐处理相比，BS处理使每个反应中心的电子传递速率（ET/RC）降低了10%，盐处理显示出较少的反应中心损伤。在胁迫条件下接种芽孢杆菌和种子引发处理（PBS）使电子传递速率提高了40%。盐胁迫在12天后开始对PSII产生显著影响，并在22天后对所有形态和光合参数产生不利影响。盐引发和PGPB减轻了盐胁迫的负面影响，可能成为保障盐碱农业未来发展的有效工具。
