Song Yang, Cui Yibo, Zhong Yue, Wang Yumeng, Zheng Xin
College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Road, Nanguan District, Changchun, Jilin 130118, China.
College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Road, Nanguan District, Changchun, Jilin 130118, China.
J Biotechnol. 2025 Mar;399:81-90. doi: 10.1016/j.jbiotec.2025.01.012. Epub 2025 Jan 16.
Modern management of chicks hinders the vertical transmission of intestinal microbiota, which is closely related to immunity. Inulin is a substrate that can be utilized by the microbiota. This study aimed to determine whether fecal microbiota transplantation (FMT) combined with inulin played a "1 + 1 > 2" role in enhancing the development and function of immune organs. Chicks were treated with 1 % inulin and/or fecal microbiota suspension on days 1-6. The growth performance, immune organ development, and immune indicators were evaluated on days 7, 14, and 21. Results showed that the combination of FMT and inulin significantly increased the immune organ index on day 7 and promoted the morphological structure and the expression of proliferating cell nuclear antigen (PCNA) in immune organs on days 7, 14, and 21. Each treatment increased the gene expression of interferon-gamma (IFN-γ), interleukin-4 (IL-4), interleukin-2 (IL-2), B cell-activating factor receptor (BAFFR), B cell linker (BLNK), C-X-C Motif Chemokine Ligand 12 (CXCL12), C-X-C Motif Chemokine Receptor 4 (CXCR4), and Biotin (Bu-1) to varying degrees. FMT combined with inulin significantly increased the expression of IgA-positive cells on days 7 and 14. In conclusion, the synergistic effect of FMT and inulin had beneficial impacts on the development and function of immune organs.
现代雏鸡管理方式会阻碍肠道微生物群的垂直传播，而肠道微生物群与免疫力密切相关。菊粉是一种可被微生物群利用的底物。本研究旨在确定粪便微生物群移植（FMT）联合菊粉在增强免疫器官发育和功能方面是否发挥了“1 + 1 > 2”的作用。在第1至6天用1%菊粉和/或粪便微生物群悬液处理雏鸡。在第7、14和21天评估生长性能、免疫器官发育和免疫指标。结果表明，FMT与菊粉联合使用在第7天显著提高了免疫器官指数，并在第7、14和21天促进了免疫器官的形态结构和增殖细胞核抗原（PCNA）的表达。每种处理均不同程度地增加了干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）、白细胞介素-2（IL-2）、B细胞活化因子受体（BAFFR）、B细胞连接蛋白（BLNK）、C-X-C基序趋化因子配体12（CXCL12）、C-X-C基序趋化因子受体4（CXCR4）和生物素（Bu-1）的基因表达。FMT与菊粉联合使用在第7天和14天显著增加了IgA阳性细胞的表达。总之，FMT与菊粉的协同作用对免疫器官的发育和功能产生了有益影响。
