Gherezghiher T, Koss M C
Ophthalmic Res. 1985;17(2):65-71. doi: 10.1159/000265352.
An assay technique that utilizes liquid chromatography with electrochemical detection to determine the norepinephrine (NE) concentration of the rabbit aqueous humor (AH) and iris-ciliary body (ICB) is discussed. NE concentration was monitored in control preparations as well as in experiments involving stimulation of the cervical sympathetic nerve (CSN) or 24 h following extirpation of the superior cervical ganglion. In 15 control rabbits, the baseline AH NE concentration was determined to be 7.2 and 7.8 ng/ml in the left and right eyes, respectively. Prolonged unilateral electrical stimulation of the CSN increased the AH NE concentration to 59.0 +/- 1.2 ng/ml on the stimulated side. Pretreatment with cocaine (12.5 mg/kg) and subsequent stimulation of the CSN increased the NE in the AH to 93.7 +/- 1.3 ng/ml on the stimulated side, and raised the baseline AH NE of the control (non-stimulated) side to 25.9 +/- 5.0 ng/ml. The baseline concentration of NE in the ICB was 5.4 +/- 0.8 micrograms/g. 24 h after a unilateral removal of the superior cervical ganglion, the NE content of the ICB was depleted by more than 90% in the ganglionectomized eye with no apparent change in the level of NE in the AH. The accurate analysis of catecholamines in the AH, ICB and other parts of the eye may be useful in providing information as to the extent of innervation and the possible role of sympathetic nerve supply in the overall control of AH dynamics.(ABSTRACT TRUNCATED AT 250 WORDS)
本文讨论了一种利用液相色谱-电化学检测技术来测定兔眼房水（AH）和虹膜睫状体（ICB）中去甲肾上腺素（NE）浓度的分析方法。在对照制剂以及涉及刺激颈交感神经（CSN）或颈上神经节切除术后24小时的实验中监测NE浓度。在15只对照兔中，测定左眼和右眼的基线AH NE浓度分别为7.2和7.8 ng/ml。对CSN进行长时间单侧电刺激可使刺激侧的AH NE浓度增加至59.0±1.2 ng/ml。用可卡因（12.5 mg/kg）预处理并随后刺激CSN可使刺激侧的AH中NE增加至93.7±1.3 ng/ml，并使对照（未刺激）侧的基线AH NE升高至25.9±5.0 ng/ml。ICB中NE的基线浓度为5.4±0.8微克/克。单侧切除颈上神经节24小时后，去神经节切除眼的ICB中NE含量减少了90%以上，而AH中NE水平无明显变化。准确分析AH、ICB和眼的其他部位中的儿茶酚胺可能有助于提供有关神经支配程度以及交感神经供应在AH动力学总体控制中可能作用的信息。（摘要截短至250字）
