Kulkarni Kaustubh H, Monjok Emmanuel M, Zeyssig Robert, Kouamou Ghislaine, Bongmba Odelia N, Opere Catherine A, Njie Ya Fatou, Ohia Sunny E
Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204, USA.
Neurochem Res. 2009 Mar;34(3):400-6. doi: 10.1007/s11064-008-9793-7. Epub 2008 Jul 16.
In the present study, we investigated the pharmacological action of hydrogen sulfide (H2S, using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on sympathetic neurotransmission from isolated, superfused porcine iris-ciliary bodies. We also examined the effect of H2S on norepinephrine (NE), dopamine and epinephrine concentrations in isolated porcine anterior uvea. Release of [3H]NE was triggered by electrical field stimulation and basal catecholamine concentrations was measured by high performance liquid chromatography (HPLC). Both NaHS and Na2S caused a concentration-dependent inhibition of electrically evoked [3H]NE release from porcine iris-ciliary body without affecting basal [3H]NE efflux. The inhibitory action of H2S donors on NE release was attenuated by aminooxyacetic acid (AOA) and propargyglycine (PAG), inhibitors of cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE), respectively. With the exception of dopamine, NaHS caused a concentration-dependent reduction in endogenous NE and epinephrine concentrations in isolated iris-ciliary bodies. We conclude that H2S can inhibit sympathetic neurotransmission from isolated porcine anterior uvea, an effect that is dependent, at least in part, on intramural biosynthesis of this gas. Furthermore, the observed action of H2S donors on sympathetic transmission may be due to a direct action of this gas on neurotransmitter pools.
在本研究中，我们研究了硫化氢（H₂S，使用氢硫化钠、NaHS和/或硫化钠、Na₂S作为供体）对分离的、经超灌注的猪虹膜睫状体交感神经传递的药理作用。我们还研究了H₂S对分离的猪眼前葡萄膜中去甲肾上腺素（NE）、多巴胺和肾上腺素浓度的影响。通过电场刺激触发[³H]NE的释放，并通过高效液相色谱法（HPLC）测量基础儿茶酚胺浓度。NaHS和Na₂S均导致猪虹膜睫状体电场诱发的[³H]NE释放呈浓度依赖性抑制，而不影响基础[³H]NE外流。硫化氢供体对NE释放的抑制作用分别被氨基氧乙酸（AOA）和炔丙基甘氨酸（PAG）减弱，它们分别是胱硫醚β合酶（CBS）和胱硫醚γ裂解酶（CSE）的抑制剂。除多巴胺外，NaHS导致分离的虹膜睫状体中内源性NE和肾上腺素浓度呈浓度依赖性降低。我们得出结论，H₂S可以抑制分离的猪眼前葡萄膜的交感神经传递，这种作用至少部分取决于该气体的壁内生物合成。此外，观察到的硫化氢供体对交感神经传递的作用可能是由于该气体对神经递质池的直接作用。
