通过自体荧光测量对蓝绿光的晶状体透射率进行体内评估。
In vivo assessment of lens transmission for blue-green light by autofluorescence measurement.
作者信息
van Best J A, Tsoi E W, Boot J P, Oosterhuis J A
出版信息
Ophthalmic Res. 1985;17(2):90-5. doi: 10.1159/000265356.
A simple quantitative and reproducible method for evaluating lens transmission (lambda = 490 and 530 nm) is described. It is based on the measurement of autofluorescence in the anterior and the posterior part of the lens by means of a fluorophotometer, assuming an about equal fluorescence quantum efficiency in both parts. Consequently any difference in fluorescence between both parts can be attributed to a loss of exciting and fluorescent light in the lens. For both wavelengths, the average lens transmission is determined by this method in a normal population as a function of age.
描述了一种用于评估晶状体透光率（波长 = 490 和 530 纳米）的简单定量且可重复的方法。该方法基于通过荧光光度计测量晶状体前部和后部的自发荧光，并假设两部分的荧光量子效率大致相等。因此，两部分之间荧光的任何差异都可归因于晶状体中激发光和荧光的损失。对于这两个波长，通过此方法在正常人群中确定平均晶状体透光率随年龄的变化。
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