Li Qiao, Wang Yunhao, Jiang Siyu, Xie Shumin, Wu Feng, Zeng Xiangyin, Li Sen, Dai Zhongquan, Yan Qiuxin, Wang Jinpeng, Hou Xiaoyu, Yang Fan, Pi Yaning, Zhang Manrui, Diao Yan, Wei Lijun
School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
State Key Laboratory of Space Medicine, China Astronaut Research and Training Center, China.
Int J Biol Macromol. 2025 Apr;299:140006. doi: 10.1016/j.ijbiomac.2025.140006. Epub 2025 Jan 17.
This study explores the extraction and purification of polysaccharides from Dictyophora indusiata (D. indusiata) with its antioxidant and anti-weightlessness bone loss properties. An anionic polysaccharide (SADIP) with a molecular weight of 13.42 kDa was isolated and purified from D. indusiata. SADIP consists of a glucose-based sugar chain backbone. Its backbone consists of glycosidic linkages of →4)-α-D-Glcp-(1 → and →4)-β-D-Glcp-(1→, with sulfate groups attached at the O-6 position of →4)-β-D-Glcp-(1 → residues. Side chains were α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → linked at C-6 position of →4,6)-α-D-Glcp-(1 → through O-6 bonds. In vitro, SADIP scavenged up to 99.32 ± 0.24 % of 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals and 76.72 ± 0.34 % of hydroxyl radicals. In vivo, SADIP reduced malondialdehyde (MDA) levels induced by weightlessness and increased low levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) caused by weightlessness. Additionally, three-point mechanical bending tests and Micro-CT analysis demonstrated that SADIP has ability to alleviate bone loss induced by weightlessness. This study provides a theoretical foundation for the rational developmation of D. indusiata resources.
本研究探索了从竹荪中提取和纯化多糖及其抗氧化和抗失重性骨质流失特性。从竹荪中分离并纯化出一种分子量为13.42 kDa的阴离子多糖（SADIP）。SADIP由基于葡萄糖的糖链骨架组成。其骨架由→4)-α-D-葡萄糖-(1→和→4)-β-D-葡萄糖-(1→的糖苷键组成，硫酸基团连接在→4)-β-D-葡萄糖-(1→残基的O-6位。侧链是通过O-6键在→4,6)-α-D-葡萄糖-(1→的C-6位连接的α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→。在体外，SADIP清除高达99.32±0.24%的2,2'-偶氮双-(3-乙基苯并噻唑啉-6-磺酸盐)（ABTS）自由基和76.72±0.34%的羟基自由基。在体内，SADIP降低了失重诱导的丙二醛（MDA）水平，并提高了失重导致的低水平超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）。此外，三点机械弯曲试验和Micro-CT分析表明，SADIP有能力减轻失重诱导的骨质流失。本研究为竹荪资源的合理开发提供了理论基础。
