Ye Lei, Huang Yu, Zhang Lingzi, Li Xin, Zhang Bo, Yang Xuezhen, Luo Jianhua, Liu Hongping, Zhang Xiaoping, Song Chuan, Ao Zonghua, Shen Caihong, Tan Wei, Li Xiaolin
Sichuan Institute of Edible Fungi, Chengdu 610066, China; College of Resources, Sichuan Agricultural University, Chengdu 611130, China; Sichuan Jindi Fungus Co., Ltd., Chengdu 610066, China.
Sichuan Institute of Edible Fungi, Chengdu 610066, China.
Int J Biol Macromol. 2025 Jan;284(Pt 1):138149. doi: 10.1016/j.ijbiomac.2024.138149. Epub 2024 Nov 28.
An acidic polysaccharose (YL-D2N2) was isolated from crude polysaccharides of pink Auricularia cornea and characterized for its structural and antioxidant properties. YL-D2N2 consists of fucose, galactose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.85: 1.50: 4.44: 27.52: 46.56: 19.13. It has a number-average molecular weight of about 52.811 kDa and a weight-average molecular weight of about 135.457 kDa. Structural characterization showed that YL-D2N2 consists of nine residues (Xylp-(1→, GlcpA-(1→, →2)-Xylp-(1→, →3)-Galp-(1→, →3)-Manp-(1→, →4)-GlcpA-(1→, →2,3)-Manp-(1→, →3,4)-Glcp-(1→, →3,6)-Manp-(1→), with a backbone of →3)-β-D-Manp-(1→, →2,3)-α-D-Manp-(1→, →3,6)-α-D-Manp-(1 → and side chains containing β-D-Xylp-(1 → and α-D-GlcpA-(1→. Notably, YL-D2N2 exhibits significant radical scavenging activity for superoxide anions, reaching 50.82 ± 0.64 % at a concentration of 3.2 mg/mL. Overall, YL-D2N2 exhibits a unique chemical structure and specialized applications for targeting superoxide anion radicals, providing valuable insights for further exploration of its structure-activity relationship.
从粉红木耳粗多糖中分离出一种酸性多糖（YL-D2N2），并对其结构和抗氧化性能进行了表征。YL-D2N2由岩藻糖、半乳糖、葡萄糖、木糖、甘露糖和葡萄糖醛酸组成，摩尔比为0.85:1.50:4.44:27.52:46.56:19.13。其数均分子量约为52.811 kDa，重均分子量约为135.457 kDa。结构表征表明，YL-D2N2由九个残基组成（木糖基-(1→，葡萄糖醛酸基-(1→，→2)-木糖基-(1→，→3)-半乳糖基-(1→，→3)-甘露糖基-(1→，→4)-葡萄糖醛酸基-(1→，→2,3)-甘露糖基-(1→，→3,4)-葡萄糖基-(1→，→3,6)-甘露糖基-(1→），主链为→3)-β-D-甘露糖基-(1→，→2,3)-α-D-甘露糖基-(1→，→3,6)-α-D-甘露糖基-(1→，侧链含有β-D-木糖基-(1→和α-D-葡萄糖醛酸基-(1→。值得注意的是，YL-D2N2对超氧阴离子具有显著的自由基清除活性，在浓度为3.2 mg/mL时达到50.82±0.64%。总体而言，YL-D2N2具有独特的化学结构和针对超氧阴离子自由基的特殊应用，为进一步探索其构效关系提供了有价值的见解。
