Wang Tianbao, Qin Yufei, Wang Jin-Yu, Xu Yihan, Guo Jiaming, Zhu Yiling, Zhang Huiyan, Qin Yujie, Qi Zhong-Quan, Fu Hualong, Liu Ya-Jun, Cui Mengchao, Zhou Kaixiang
Center for Advanced Materials Research & Faculty of Arts and Sciences, Beijing Normal University, Zhuhai 519087, China.
Medical College, Guangxi University, Nanning, Guangxi 530004, China.
J Med Chem. 2025 Feb 13;68(3):3782-3794. doi: 10.1021/acs.jmedchem.4c02866. Epub 2025 Jan 19.
Fluorescence molecular imaging aims to enhance clarity in the region of interest, particularly in the near-infrared IIb window (NIR-IIb, 1500-1700 nm). To achieve this, we developed a novel small-molecule dye, named , based on classic cyanine dyes (heptamethine or pentamethine is essential for wavelengths beyond 1000 nm). By reducing excessive polymethine to a single methine and disrupting symmetry to form an asymmetric donor-π-acceptor (D-π-A) architecture, we enhanced the donor's electron-donating capability, yielding emission at 1088 nm. exhibits superior properties, including excellent chemo- and photostability, resistance against solvatochromism-caused quenching, and antiaggregation in aqueous solution. With a large Stokes shift (241 nm) and high brightness (321 M cm), enables high-performance imaging of the lymphatic system, intestinal vessels, whole-body angiography, and cerebral and hindlimb microvasculature in NIR-IIb. This molecular design strategy offers a promising platform for advancing in vivo biophotonics.
荧光分子成像旨在提高感兴趣区域的清晰度，特别是在近红外IIb窗口（NIR-IIb，1500 - 1700纳米）。为实现这一目标，我们基于经典的花青染料（对于波长超过1000纳米，七甲川或五甲川至关重要）开发了一种名为 的新型小分子染料。通过将过多的聚甲川还原为单个甲川并破坏对称性以形成不对称供体-π-受体（D-π-A）结构，我们增强了供体的给电子能力，从而在1088纳米处产生发射。 具有卓越的性能，包括出色的化学稳定性和光稳定性、抗溶剂化显色引起的猝灭能力以及在水溶液中的抗聚集性。凭借大斯托克斯位移（241纳米）和高亮度（321 M cm）， 能够在NIR-IIb中对淋巴系统、肠道血管、全身血管造影以及脑和后肢微血管进行高性能成像。这种分子设计策略为推进体内生物光子学提供了一个有前景的平台。
