Dinana Zayyin, Suwarno Suwarno, Mustofa Imam, Rahmahani Jola, Kusnoto Kusnoto, Maharani Aussie Tahta, Fitria Anisa Lailatul, Witaningrum Adiana Mutamsari, Maulana Firdausy Kurnia, Said Nur Saidah, Fahrodi Deka Uli, Rantam Fedik Abdul
Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Viral Diarrhea, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2024 Nov;17(11):2469-2476. doi: 10.14202/vetworld.2024.2469-2476. Epub 2024 Nov 7.
Foot and mouth disease (FMD) is highly contagious in cloven-hoofed animals, and it causes outbreaks in Indonesia and several countries worldwide. This disease is caused by the FMD virus (FMDV), which belongs to the genus Aphthovirus and family Picornaviridae. In 1990, the World Organization for Animal Health Office International des Epizooties recognized Indonesia as an FMD-free country. A new FMDV outbreak in Indonesia was reported in April 2022 and confirmed in May 2022, resulting in economic losses to the beef cattle sector. This study aimed to determine the genotype and amino acid content of viral proteins () gene.
Samples were obtained from vesicle swabs from the mouth and feet of cattle in Banyuwangi Regency, Lamongan Regency, and Surabaya City, East Java, Indonesia. Samples were identified using one-step reverse transcriptase-polymerase chain reaction with a pair of specific primers encoding the O serotype with a target of 1165 bp.
Sequencing revealed that the FMDV subtype belonged to the O/ME-SA/Ind2001e. Phylogenetic analysis showed that our isolate was 100% amino acid-identical to the Indonesian outbreak isolates from 2022 and 95% identical to isolates from Southeast Asia. The amino acid substitutions found in the G-H Loop of the were S134C, D138E, T140A, and A156T. Only the K135Q mutation was detected in Lamongan.
The spread of the subtype O/ME-SA/Ind2001e in South-east Asia caused an outbreak in Indonesia due to less stringent animal traffic control measures. Surveillance studies and whole-genome sequence analyses are important for monitoring FMDV genetics in Indonesia.
口蹄疫（FMD）在偶蹄动物中具有高度传染性，在印度尼西亚和世界上其他几个国家引发了疫情。这种疾病由口蹄疫病毒（FMDV）引起，该病毒属于口疮病毒属，科为小核糖核酸病毒科。1990年，世界动物卫生组织国际兽疫局认定印度尼西亚为无口蹄疫国家。2022年4月，印度尼西亚报告了一起新的口蹄疫病毒疫情，并于2022年5月得到确认，给肉牛产业造成了经济损失。本研究旨在确定病毒蛋白（）基因的基因型和氨基酸含量。
样本取自印度尼西亚东爪哇省班尤万吉县、拉蒙甘县和泗水市牛的口腔和蹄部水疱拭子。使用一步逆转录聚合酶链反应，用一对编码O血清型的特异性引物进行样本鉴定，目标片段为1165 bp。
测序显示口蹄疫病毒亚型属于O/ME-SA/Ind2001e。系统发育分析表明，我们分离出的毒株与2022年印度尼西亚疫情分离株的氨基酸序列100%相同，与东南亚分离株的氨基酸序列95%相同。在的G-H环中发现的氨基酸替换为S134C、D138E、T140A和A156T。在拉蒙甘仅检测到K135Q突变。
由于动物运输控制措施不够严格，O/ME-SA/Ind2001e亚型在东南亚的传播导致了印度尼西亚的疫情爆发。监测研究和全基因组序列分析对于监测印度尼西亚口蹄疫病毒的遗传学特征非常重要。
