Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh.
Research on FMD and PPR in Bangladesh, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh.
Open Vet J. 2020 Oct;10(3):347-353. doi: 10.4314/ovj.v10i3.14. Epub 2020 Sep 30.
Foot-and-mouth disease (FMD) is an endemic disease of cloven-hoofed animals in Bangladesh and multiple outbreaks occur every year because of the FMD virus (FMDV).
The aim of the present investigation was to determine the molecular characterization of the VP1 coding region of FMDV serotype O outbreak in cattle.
A total of four tongue epithelial specimens were collected from clinically FMD-positive cattle during June 2018 in Manikgonj district of Bangladesh.
All four isolates were recorded positive for FMDV serotype O. The phylogenetic analysis showed that two isolates were clustered within an emerging novel sublineage Ind2001BD1 under lineage Ind2001 of FMDV serotype O, which was identified during 2012-2016 in Bangladesh. One isolate was clustered within the lineage PanAsia of FMDV serotype O and was closely related to an isolate identified in Nepal in 2009. The phylogenetic reconstruction revealed that all the four isolates belong to the Middle East-South Asia topotype.
Therefore, multiple lineages of the FMDV serotype O are circulating among the cattle in the outbreak area, which make it more complex for the FMD control program in Bangladesh. A comprehensive study on the genetic characteristics of FMDV across the country is required for effective FMD prevention and control strategy.
口蹄疫（FMD）是孟加拉国偶蹄类动物的地方性疾病，由于口蹄疫病毒（FMDV）的存在，每年都会多次爆发。
本研究旨在确定孟加拉国马尼岗地区 2018 年 6 月牛群中暴发的口蹄疫病毒血清型 O 的 VP1 编码区的分子特征。
从临床上患有 FMD 的牛的舌上皮组织中采集了 4 个舌上皮标本。
所有 4 个分离株均被记录为 FMDV 血清型 O 阳性。系统进化分析表明，有 2 个分离株聚集在 FMDV 血清型 O 的 Ind2001 谱系内的新兴新型亚谱系 Ind2001BD1 中，该亚谱系于 2012-2016 年在孟加拉国被鉴定。有 1 个分离株聚集在 FMDV 血清型 O 的泛亚谱系中，与 2009 年在尼泊尔鉴定的分离株密切相关。系统发育重建显示，所有 4 个分离株均属于中东-南亚拓扑型。
因此，在疫区牛群中存在多种血清型 O 的 FMDV 谱系，这使得孟加拉国的 FMD 控制计划更加复杂。需要对全国范围内的 FMDV 遗传特征进行综合研究，以制定有效的 FMD 防控策略。
