Shukla Yash, Ghatpande Vighnesh, Hu Cindy F, Dickinson Daniel J, Cenik Can
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
bioRxiv. 2025 Apr 1:2024.12.13.628416. doi: 10.1101/2024.12.13.628416.
Animal embryos rely on regulated translation of maternally deposited mRNAs to drive early development. Using low-input ribosome profiling combined with RNA sequencing on precisely staged embryos, we measured mRNA translation during the first four cell cycles of development. We uncovered stage-specific patterns of developmentally coordinated translational regulation. We confirmed that mRNA localization correlates with translational eLiciency, though initial translational repression in germline precursors occurs before P-granule association. Our analysis suggests that the RNA-binding protein OMA-1 represses the translation of its target mRNAs in a stage-specific manner, while indirectly promoting the translational eLiciency of other transcripts. These findings illuminate how post-transcriptional mechanisms shape the embryonic proteome to direct cell diLerentiation, with implications for understanding similar regulation across species where maternal factors guide early development.
动物胚胎依靠对母源沉积mRNA的翻译调控来驱动早期发育。我们运用低投入核糖体谱分析技术，并结合对精确分期胚胎的RNA测序，测量了发育前四个细胞周期中的mRNA翻译情况。我们发现了发育协调的翻译调控的阶段特异性模式。我们证实，mRNA定位与翻译效率相关，尽管种系前体中的初始翻译抑制在P颗粒结合之前就已发生。我们的分析表明，RNA结合蛋白OMA-1以阶段特异性方式抑制其靶mRNA的翻译，同时间接促进其他转录本的翻译效率。这些发现阐明了转录后机制如何塑造胚胎蛋白质组以指导细胞分化，这对于理解母体因素指导早期发育的跨物种类似调控具有重要意义。
