Shukla Yash, Ghatpande Vighnesh, Hu Cindy F, Dickinson Daniel J, Cenik Can
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Cell Rep. 2025 May 30;44(6):115778. doi: 10.1016/j.celrep.2025.115778.
Animal embryos rely on regulated translation of maternally deposited mRNAs to drive early development. Using low-input ribosome profiling combined with RNA sequencing on precisely staged embryos, we measure mRNA translation during the first four cell cycles of C. elegans development. We uncover stage-specific patterns of developmentally coordinated translational regulation. Our results confirm that mRNA localization correlates with translational efficiency, though initial translational repression in germline precursors occurs before P-granule association. Our analysis suggests that the RNA-binding protein OMA-1 represses the translation of its target mRNAs in a stage-specific manner while indirectly promoting the translational efficiency of other transcripts. These findings illuminate how post-transcriptional mechanisms shape the embryonic proteome to direct cell differentiation, with implications for understanding similar regulation across species where maternal factors guide early development.
动物胚胎依靠母源沉积mRNA的翻译调控来驱动早期发育。我们在精确分期的胚胎上结合RNA测序使用低输入核糖体图谱分析，测量秀丽隐杆线虫发育前四个细胞周期中的mRNA翻译。我们发现了发育协调翻译调控的阶段特异性模式。我们的结果证实，mRNA定位与翻译效率相关，尽管种系前体中的初始翻译抑制发生在P颗粒结合之前。我们的分析表明，RNA结合蛋白OMA-1以阶段特异性方式抑制其靶mRNA的翻译，同时间接促进其他转录本的翻译效率。这些发现阐明了转录后机制如何塑造胚胎蛋白质组以指导细胞分化，对理解母体因素指导早期发育的跨物种类似调控具有启示意义。
