Chung Hung Hui, Soh Asmeralda Ai Leen, Lau Melinda Mei Lin, Gan Han Ming, Sim Siong Fong, Lim Leonard Whye Kit
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Patriot Biotech Sdn Bhd, 47500 Subang Jaya, Selangor, Malaysia.
Data Brief. 2024 Dec 20;58:111248. doi: 10.1016/j.dib.2024.111248. eCollection 2025 Feb.
The engkabang jantong () is one of the most indispensable tree species for reforestation due to its high survival rate and rapid growth rate. Due to relatively low genetic interest of this tree species, its genomic landscape has since faced scarcity, impeding our further elucidation on genes that are involved in expressing its aforementioned superior properties. In this study, we performed genome survey and microsatellite analysis of engkabang jantong. Based on the results, the estimated genome size of this species is 312,071,515 bp with 18.43 % repeated sequences and 1.16 % heterozygosity. BUSCO analysis unearthed that 83.5 % of the contigs are single-copy genes whereas 12.7 % of them are duplicated. Only 2.8 % and 1 % of them are fragmented and missing respectively. The short-read sequencing results obtained from the Illumina platform in this study will be essential to complement the Nanopore long-read sequencing results in hybrid genome assembly endeavors in the near future.
由于其高成活率和快速生长率，红厚壳（engkabang jantong ()）是重新造林中最不可或缺的树种之一。由于该树种的遗传研究兴趣相对较低，其基因组图谱一直面临稀缺，阻碍了我们对参与表达其上述优良特性的基因的进一步阐明。在本研究中，我们对红厚壳进行了基因组调查和微卫星分析。基于结果，该物种的估计基因组大小为312,071,515 bp，重复序列占18.43%，杂合度为1.16%。BUSCO分析发现，83.5%的重叠群是单拷贝基因，而其中12.7%是重复的。它们中分别只有2.8%和1%是片段化和缺失的。本研究从Illumina平台获得的短读长测序结果对于在不久的将来补充纳米孔长读长测序结果用于混合基因组组装工作至关重要。
