Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
GeneSEQ Sdn Bhd, Bukit Beruntung, 48300 Rawang, Selangor, Malaysia; Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
Gene. 2021 Jul 30;791:145708. doi: 10.1016/j.gene.2021.145708. Epub 2021 May 11.
The true mahseer (Tor spp.) is one of the highest valued fish in the world due to its high nutritional value and great unique taste. Nevertheless, its morphological characterization and single mitochondrial gene phylogeny in the past had yet to resolve the ambiguity in its taxonomical classification. In this study, we sequenced and assembled 11 complete mahseer mitogenomes collected from Java of Indonesia, Pahang and Terengganu of Peninsular Malaysia as well as Sarawak of East Malaysia. The mitogenome evolutionary relationships among closely related Tor spp. samples were investigated based on maximum likelihood phylogenetic tree construction. Compared to the commonly used COX1 gene fragment, the complete COX1, Cytb, ND2, ND4 and ND5 genes appear to be better phylogenetic markers for genetic differentiation at the population level. In addition, a total of six population-specific mitolineage haplotypes were identified among the mahseer samples analyzed, which this offers hints towards its taxonomical landscape.
真正的巨魾（Tor spp.）因其高营养价值和独特的口感而成为世界上最具价值的鱼类之一。然而，过去其形态特征和单一线粒体基因系统发育尚未解决其分类学分类中的模糊性。在这项研究中，我们从印度尼西亚爪哇岛、马来西亚半岛的彭亨州和登嘉楼州以及东马来西亚的沙捞越州收集了 11 个完整的巨魾线粒体基因组并进行了测序和组装。基于最大似然系统发育树构建，研究了密切相关的 Tor spp.样本之间的线粒体进化关系。与常用的 COX1 基因片段相比，完整的 COX1、Cytb、ND2、ND4 和 ND5 基因似乎是种群水平遗传分化的更好的系统发育标记。此外，在所分析的巨魾样本中总共鉴定出 6 种种群特异性的线粒体谱系单倍型，这为其分类景观提供了线索。
