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通风口叶片对人乳腺癌细胞系（MCF-7）的抗增殖和促凋亡活性

Antiproliferative and Pro-apoptotic Activities of vent. Leaves on the Human Breast Adenocarcinoma Cell Line (MCF-7).

作者信息

Mora-Guzmán Zoila, Bernardo Flores-Cotera Luis, Pérez-Campos Eduardo, López-Marure Rebeca, Soto-Castro Delia, Alonso Masso-Rojas Felipe, Paéz Arenas Araceli, Zenteno Edgar, Martinez-Cruz Margarito, Pérez-Campos Mayoral Laura, Hernández-Huerta María Teresa, Pina-Canseco María Del Socorro

机构信息

UNAM-UABJO, Faculty of Medicine Research Center, Faculty of Medicine and Surgery, Autonomous University Benito Juarez of Oaxaca, Oaxaca, Mexico.

Department of Biotechnology and Bioengineering, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Oaxaca, Mexico.

出版信息

Iran J Pharm Res. 2024 Sep 16;23(1):e149405. doi: 10.5812/ijpr-149405. eCollection 2024 Jan-Dec.

DOI:10.5812/ijpr-149405
PMID:39830672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11742378/
Abstract

BACKGROUND

Breast cancer is the most common cancer among women worldwide, impacting not only the patients but also their families and communities. vent. is a plant endemic to Mexico, traditionally used in Zapotec medicine for the treatment of cancer.

OBJECTIVES

This study aims to evaluate the effects of the chloroformic extract of vent. leaves on cell proliferation and cell death in MCF-7 cells.

METHODS

The effect of the extract on MCF-7 cell proliferation was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet staining. Apoptosis was evaluated through fluorescein diacetate/propidium iodide staining and caspase-3, -6, and -9 activity assays.

RESULTS

The half-maximal inhibitory concentration (IC50) of the vent. extract on MCF-7 cell proliferation at 48 hours and 72 hours after treatment was 86.4 µg/mL and 2.74 µg/mL, respectively. We observed that the extract and its semi-purified fractions induced cell death through the activation of caspases 3, -6, and -9.

CONCLUSIONS

vent. is a potential source of compounds with antiproliferative and pro-apoptotic activities on the MCF-7 cell line, primarily through the intrinsic pathways of apoptosis.

摘要

背景

乳腺癌是全球女性中最常见的癌症，不仅影响患者，还影响其家庭和社区。vent.是一种墨西哥特有的植物，在萨波特克医学中传统上用于治疗癌症。

目的

本研究旨在评估vent.叶氯仿提取物对MCF-7细胞增殖和细胞死亡的影响。

方法

使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和结晶紫染色测量提取物对MCF-7细胞增殖的影响。通过荧光素二乙酸酯/碘化丙啶染色和半胱天冬酶-3、-6和-9活性测定评估细胞凋亡。

结果

vent.提取物在处理后48小时和72小时对MCF-7细胞增殖的半数抑制浓度（IC50）分别为86.4μg/mL和2.74μg/mL。我们观察到提取物及其半纯化级分通过激活半胱天冬酶3、-6和-9诱导细胞死亡。

结论

vent.是对MCF-7细胞系具有抗增殖和促凋亡活性的化合物的潜在来源，主要通过凋亡的内在途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/9ab82a33a7ad/ijpr-23-1-149405-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/22113802d335/ijpr-23-1-149405-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/310e5775c48f/ijpr-23-1-149405-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/797c8b1b36fa/ijpr-23-1-149405-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/542f893eefce/ijpr-23-1-149405-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/fa8b35d4bd12/ijpr-23-1-149405-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/9ab82a33a7ad/ijpr-23-1-149405-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/22113802d335/ijpr-23-1-149405-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/310e5775c48f/ijpr-23-1-149405-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/797c8b1b36fa/ijpr-23-1-149405-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/542f893eefce/ijpr-23-1-149405-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/fa8b35d4bd12/ijpr-23-1-149405-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d663/11742378/9ab82a33a7ad/ijpr-23-1-149405-g002.jpg

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