Nikolaeva M Ia, Balmukhanov B S, Parkhimovich R M
Probl Endokrinol (Mosk). 1985 Jan-Feb;31(1):14-8.
It is known that dehydroascorbic acid (DHAA) produces a diabetogenic effect and its content in the blood increases in diabetes mellitus. It was previously established that the generation of reducing equivalents (RE) in the course of hexosemonophosphate shunt, CO2 production and SH-glutathione regeneration in erythrocytes with and without moderate and maximum oxidation load in vitro were not disturbed in diabetes. The authors have proposed a procedure to study blood and erythrocyte DHAA reductase activity in suspension in health and in insulin-dependent diabetes mellitus by means of redoxstatometry using a device of original design. A significant acceleration of RE transfer through the erythrocyte membrane was detected in diabetes. A lowered participation in this process of the AA in equilibrium DHAA "shuttle" system was recorded in the blood of patients with diabetes mellitus what was mostly expressed under the conditions of acidosis in vitro. Probably "shuttle" function in diabetes was provided by some other redox system which might be located in the plasma. The predominant functioning of this redox system and a decrease of DHAA reductase activity in diabetes resulted in the accumulation of DHAA in the blood of patients with type I diabetes mellitus.
已知脱氢抗坏血酸（DHAA）具有致糖尿病作用，且其在血液中的含量在糖尿病患者中会升高。此前已证实，在体外有或无中度及最大氧化负荷的情况下，糖尿病患者红细胞中磷酸己糖旁路过程中还原当量（RE）的生成、二氧化碳的产生以及SH-谷胱甘肽的再生均未受到干扰。作者提出了一种通过使用原始设计的装置进行氧化还原电位测定法来研究健康人和胰岛素依赖型糖尿病患者血液及悬浮红细胞中DHAA还原酶活性的方法。在糖尿病患者中检测到RE通过红细胞膜的转移显著加速。在糖尿病患者的血液中，平衡的DHAA“穿梭”系统中抗坏血酸（AA）参与该过程的程度降低，这在体外酸中毒条件下表现得最为明显。糖尿病中的“穿梭”功能可能由其他一些可能位于血浆中的氧化还原系统提供。该氧化还原系统的主要作用以及糖尿病中DHAA还原酶活性的降低导致了I型糖尿病患者血液中DHAA的积累。
