Chandler Jennifer L, Trotter Robert Talbot
Northern Research Station, U.S. Forest Service, United States Department of Agriculture, Hamden, CT, USA.
J Insect Sci. 2025 Jan 20;25(1). doi: 10.1093/jisesa/ieae119.
Asian longhorned beetle (Anoplophora glabripennis Motschulsky), a wood borer (Coleoptera: Cerambycidae) native to China, has been unintentionally and repeatedly introduced to North American and European landscapes as a stow-away in the wood packing material commonly used in international trade. Asian longhorned beetle causes extensive damage and mortality in multiple deciduous tree species and in response, countries in both North America and Europe have adopted policies of eradication. Models that integrate patterns of Asian longhorned beetle dispersal with records of infested trees are critical in optimizing survey and eradication efforts and tracking eradication progress. While these tools continue to be developed, they have been limited by the availability of experimental dispersal data. Existing data is restricted to observations made in the beetle's native range in China or based on inference of dispersal in invaded landscapes. Direct observation of beetle dispersal behavior in invaded landscapes could provide critical behavioral information, but the experimental release of gravid females has been incompatible with eradication program efforts. To fill this knowledge gap, there is a need to identify field-portable methods of effectively sterilizing mated females that do not alter ovipositional behavior. Here, we present a protocol for cauterizing a beetle's ovipositor to prevent successful oviposition. Results of lab trials demonstrate the efficacy of ovipositor cauterization in inhibiting successful oviposition without altering the egg-laying behavior of gravid Asian longhorned beetle females. This method enables research to inform models of beetle dispersal and infestation risk without adding to actual or perceived risk of exacerbating infestations in an eradication program.
亚洲长角天牛（Anoplophora glabripennis Motschulsky）是一种原产于中国的蛀木虫（鞘翅目：天牛科），作为国际贸易中常用的木质包装材料中的偷渡者，它被无意且多次引入北美和欧洲地区。亚洲长角天牛会对多种落叶树种造成广泛破坏并导致树木死亡，对此，北美和欧洲的国家都采取了根除政策。将亚洲长角天牛的扩散模式与受侵染树木的记录相结合的模型，对于优化调查和根除工作以及追踪根除进展至关重要。虽然这些工具仍在不断开发，但它们一直受到实验性扩散数据可用性的限制。现有数据仅限于在中国甲虫原生范围内的观察结果，或基于对入侵地区扩散情况的推断。直接观察甲虫在入侵地区的扩散行为可以提供关键的行为信息，但怀有身孕的雌虫的实验性释放与根除计划的努力不相容。为了填补这一知识空白，需要确定能够有效使已交配雌虫绝育且不改变其产卵行为的现场便携方法。在此，我们提出一种烧灼甲虫产卵器以防止成功产卵的方案。实验室试验结果表明，烧灼产卵器在抑制成功产卵方面是有效的，同时不会改变怀有身孕的亚洲长角天牛雌虫的产卵行为。这种方法能够让研究为甲虫扩散和侵染风险模型提供信息，而不会增加根除计划中实际或感知到的加剧侵染的风险。
