Hu Yongli, Du Yan, Qiu Zhisheng, Mao Pengxue, Da Mingxu
The First Clinical Medical College of Lanzhou University, Lanzhou University, Lanzhou 730000, China; Department of Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, China.
The Second Clinical Medical College of Lanzhou University, Lanzhou University, Lanzhou 730000, China.
Int Immunopharmacol. 2025 Feb 20;148:114047. doi: 10.1016/j.intimp.2025.114047. Epub 2025 Jan 19.
This study investigated the expression pattern of Vesicular Amine Transporter 1 (VAT1) in gastric cancer (GC) and its impact on prognosis, alongside evaluating its potential as a biomarker for immunotherapy and chemotherapy. Analysis of transcriptomic data, supported by experimental validation, revealed that VAT1 is highly expressed in GC and is associated with poor prognosis. Kaplan-Meier and ROC analyses demonstrated VAT1's potential in GC diagnosis, while multivariate analysis confirmed its role as an independent risk factor. Gene set enrichment analysis indicated that VAT1 plays a role in regulating the MAPK signaling pathway and epithelial-mesenchymal transition (EMT) in GC. Immune infiltration analysis showed a positive correlation between VAT1 and immune cells, particularly macrophages, and a negative correlation with chemotherapy sensitivity. In vitro and in vivo experiments further confirmed VAT1's critical role in promoting GC cell proliferation and inhibiting apoptosis. Overall, VAT1 holds significant value not only in GC diagnosis and prognosis but also as a potential target for immunotherapy and overcoming drug resistance.
本研究调查了囊泡胺转运体1（VAT1）在胃癌（GC）中的表达模式及其对预后的影响，同时评估其作为免疫治疗和化疗生物标志物的潜力。在实验验证的支持下，对转录组数据的分析表明，VAT1在GC中高表达且与预后不良相关。Kaplan-Meier和ROC分析证明了VAT1在GC诊断中的潜力，而多变量分析证实了其作为独立危险因素的作用。基因集富集分析表明，VAT1在调节GC中的MAPK信号通路和上皮-间质转化（EMT）中发挥作用。免疫浸润分析显示，VAT1与免疫细胞，特别是巨噬细胞呈正相关，与化疗敏感性呈负相关。体外和体内实验进一步证实了VAT1在促进GC细胞增殖和抑制凋亡中的关键作用。总体而言，VAT1不仅在GC诊断和预后中具有重要价值，而且作为免疫治疗和克服耐药性的潜在靶点也具有重要价值。
