Zhai Tong, Shen Yu-Di, Yu Miao, Liu Jing-Min, Wang Shuo
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, No. 94 Weijin Road, Tianjin, 300071, China.
Mikrochim Acta. 2025 Apr 9;192(5):289. doi: 10.1007/s00604-025-07145-6.
Robust detection techniques combined with functional micro-/nano-entities can be an ideal solution for food-safety risk treatment related to aflatoxin B1 (AFB1). In this work, a porous covalent architecture-involved luminescent smart hydrogel with dynamic cross-linking and target-responsive framework was prepared for specific determination of AFB1 with remarkable sensitivity. Different from the existing hydrogel probe, the introduction of covalent organic frameworks (COFs) enhanced the hydrophobicity of hydrogel, enabling the selective capture of AFB1 with improved affinity while excluding large matrix molecules, thus creating an anti-interference detection environment. Additionally, the pH-responsive carbon dots (CDs) embedded in the smart hydrogel contributed to a dual signal amplification, induced by deconstruction of hydrogel to release the CDs and urease-catalyzed hydrolysis reaction to increase the pH, when the AFB1 presented. In view of this signal amplification and trace enrichment strategy, this smart hydrogel can achieve robust determination of AFB1 with a detection limit of 0.03 µM as well as good specificity. The method was validated using real-world samples, demonstrating excellent accuracy and precision compared to standard HPLC methods. This work not only advances the field of AFB1 detection but also provides a versatile and cost-effective platform that can be adapted for the detection of other toxins and biomolecules, addressing global food safety and environmental monitoring challenges.
将强大的检测技术与功能性微/纳米实体相结合，可能是解决与黄曲霉毒素B1（AFB1）相关的食品安全风险问题的理想方案。在这项工作中，制备了一种具有动态交联和目标响应框架的多孔共价结构参与的发光智能水凝胶，用于以显著的灵敏度特异性测定AFB1。与现有的水凝胶探针不同，共价有机框架（COFs）的引入增强了水凝胶的疏水性，使其能够以更高的亲和力选择性捕获AFB1，同时排除大的基质分子，从而创造了一个抗干扰的检测环境。此外，嵌入智能水凝胶中的pH响应性碳点（CDs）有助于双重信号放大，当AFB1出现时，水凝胶解构释放CDs以及脲酶催化的水解反应使pH升高，从而引发双重信号放大。鉴于这种信号放大和痕量富集策略，这种智能水凝胶能够以0.03 µM的检测限对AFB1进行可靠测定，并且具有良好的特异性。该方法通过实际样品进行了验证，与标准HPLC方法相比，显示出优异的准确性和精密度。这项工作不仅推动了AFB1检测领域的发展，还提供了一个通用且经济高效的平台，可适用于检测其他毒素和生物分子，应对全球食品安全和环境监测挑战。
