Ding Zufa, Wang Cheng, Qian Yuting, Chen Wenjun, Yu Dan, Liu Yang, Yang Liandong, Wang Ying
Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Sciences, Jianghan University, Wuhan, 430056, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Sci Data. 2025 Jan 20;12(1):118. doi: 10.1038/s41597-025-04472-4.
The bluespotted cornetfish (Fistularia commersonii), a Lessepsian sprinter species, is distributed in the inter-tropical zone across the entire Indo-Pacific, ranging from the Tropical Eastern Pacific to the Red Sea. In this study, we achieve assembly of a chromosome-level genome for F. commersonii by harnessing the precision of PacBio HiFi sequencing in conjunction with the sophistication of Hi-C sequencing technologies. The assembly yields a size of 638.75 Mb, achieving a distribution rate of 99.35% across 36 chromosomes. Notably, the contig N50 value stands at 15.67 Mb, while the scaffold N50 value reaches an impressive 18.69 Mb. BUSCO analysis demonstrates a genome completeness of 96.9% for F. commersonii. Repetitive elements constitute approximately 29.61% of the genome's size. A total of 20,961 protein-coding genes are predicted, with 95.97% of them having functional annotations. This high-quality assembly provides crucial resources for future studies on the invasion mechanisms of F. commersonii, as well as for comparative genomics research on seahorses and pipefish.
蓝斑烟管鱼（Fistularia commersonii）是一种通过苏伊士运河迁移的物种，分布于整个印度-太平洋地区的热带区域，范围从热带东太平洋到红海。在本研究中，我们利用PacBio HiFi测序的精确性并结合Hi-C测序技术的先进性，完成了蓝斑烟管鱼染色体水平基因组的组装。该组装基因组大小为638.75 Mb，在36条染色体上的分布率达到99.35%。值得注意的是，重叠群N50值为15.67 Mb，而支架N50值高达18.69 Mb。BUSCO分析表明蓝斑烟管鱼的基因组完整性为96.9%。重复元件约占基因组大小的29.61%。总共预测出20,961个蛋白质编码基因，其中95.97%具有功能注释。这种高质量的组装为未来研究蓝斑烟管鱼的入侵机制以及海马和管口鱼的比较基因组学研究提供了关键资源。
