通过大规模平行测序对美洲豹丽鱼（Parachromis manguensis）进行染色体水平的基因组组装。

Chromosome-level genome assembly of Jaguar guapote (Parachromis manguensis) by massive parallel sequencing.

作者信息

Cao Jianmeng, Tong Yannan, Xiao Zhigang, Chen Huizi, Liu Zhigang

机构信息

Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.

Hainan Academy of Ocean and Fisheries Science, Haikou, 570206, China.

出版信息

Sci Data. 2025 Mar 10;12(1):411. doi: 10.1038/s41597-025-04752-z.

DOI:10.1038/s41597-025-04752-z

PMID:40064893

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11894119/

Abstract

Parachromis managuensis is a native cichlid fish from Central America and is the most commonly traded species within its genus. This study presents the first chromosome-scale genome assembly of P. managuensis using PacBio HiFi long reads and Hi-C sequencing data. The size of the P. managuensis genome is approximately 896.66 Mb, with a scaffold N50 of 38.19 Mb. The assembled genome demonstrates high quality in terms of completeness and accuracy, with a BUSCO score of 98.85% and a quality value (QV) of 50.95. A total of 888.60 Mb (99.10%) sequences were anchored to 24 pseudochromosomes. Additionally, 21,145 protein-coding genes and 325.58 Mb (~36.31%) repetitive sequences were identified. This chromosome-level genome assembly provides a crucial reference for studying the evolution and ecological adaptability of P. managuensis.

摘要

马那瓜副丽鱼是一种原产于中美洲的丽鱼科鱼类，也是其所属属中最常交易的物种。本研究利用PacBio HiFi长读长和Hi-C测序数据，首次对马那瓜副丽鱼进行了染色体水平的基因组组装。马那瓜副丽鱼基因组大小约为896.66 Mb，支架N50为38.19 Mb。组装的基因组在完整性和准确性方面表现出高质量，BUSCO评分为98.85%，质量值（QV）为50.95。共有888.60 Mb（99.10%）的序列被锚定到24条假染色体上。此外，还鉴定出21,145个蛋白质编码基因和325.58 Mb（约36.31%）的重复序列。这种染色体水平的基因组组装为研究马那瓜副丽鱼的进化和生态适应性提供了关键参考。