College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China.
Int J Mol Sci. 2024 Nov 12;25(22):12122. doi: 10.3390/ijms252212122.
As a plant-specific gene family, class III peroxidases (PODs) play an important role in plant growth, development, and stress responses. However, the POD gene family has not been systematically studied in . In this study, a total of 57 genes were identified in the genome. Subsequently, phylogenetic analysis and chromosome mapping revealed that were classified into six subgroups and were unevenly distributed across five chromosomes. The gene structure and conserved motifs indicated the potential for functional divergence among the different subgroups. Meanwhile, four were identified as tandem duplicated genes, with no evidence of segmental duplication. Using RNA-seq data from eight different tissues, multiple exhibited enhanced expression in apical and adventitious roots (ARs). Next, RNA-seq data from AR development combined with trend analysis showed that are implicated in the formation of ARs in tree peony. Through WGCNA based on RNA-seq, two key genes, , might be involved in heat tolerance via ABA and MeJA signaling. In addition, real-time quantitative PCR (qRT-PCR) analysis indicated that may play an important role in flower senescence. These findings deepened our understanding of POD-mediated AR development, heat tolerance, and petal senescence in tree peony.
作为植物特有的基因家族，III 类过氧化物酶（PODs）在植物的生长、发育和应激反应中起着重要作用。然而，在 中，POD 基因家族尚未得到系统研究。在本研究中，共在 基因组中鉴定出 57 个基因。随后，系统发育分析和染色体定位表明，这些基因被分为 6 个亚组，不均匀地分布在 5 条染色体上。基因结构和保守基序表明不同亚组之间存在潜在的功能分化。同时，鉴定出 4 个 为串联重复基因，没有发现片段重复。利用来自 8 个不同组织的 RNA-seq 数据，多个 在顶芽和不定根（ARs）中表现出增强表达。接下来，AR 发育的 RNA-seq 数据与趋势分析相结合表明， 参与牡丹不定根的形成。通过基于 RNA-seq 的 WGCNA，两个关键基因 和 可能通过 ABA 和 MeJA 信号参与耐热性。此外，实时定量 PCR（qRT-PCR）分析表明， 在花衰老过程中可能发挥重要作用。这些发现加深了我们对 POD 介导的不定根发育、耐热性和花瓣衰老的理解。
