Tang Xiao-Hu, Liu Zhi-Yan, Ren Jing-Wen, Zhang Heng, Tian Ye, Hu Jian-Xin, Sun Zhao-Lin, Luo Guang-Heng
Department of Urology Surgery, Guizhou Provincial People's Hospital, Guiyang, 550002, Guizhou Province, China.
Medical School, Guizhou University, Guiyang, 550002, Guizhou, China.
Sci Rep. 2025 Jan 22;15(1):2750. doi: 10.1038/s41598-025-87205-2.
The imbalance between estrogen and androgen may be an important mechanism of BPH, but the specific mechanism remains unclear. We used mixed sustained-release pellets made of testosterone and estradiol (T + E) to stimulate the establishment of a BPH rat model. Compared to the prostate hyperplasia rat model using only androgens, the new prostate hyperplasia rat model can be observed to have better macroscopic and pathological characteristics of prostate hyperplasia. We used RNA-seq and bioinformatics to detect differentially expressed genes (DEGs) between the prostate tissue of the novel benign prostatic hyperplasia rat group and the control group, including 458 DEGs, of which 336 were upregulated and 122 were downregulated. Then, RT-qPCR confirmed the authenticity of sequencing results. The analysis results showed that Kif4a and Mki67 were the top core genes in the PPI network. Moreover, we found that these two genes have a positive correlation with each other in multiple cancer tissues, normal tissues, and cancer cells. The DEGs were mainly involved in mitotic nuclear division, nuclear chromosome segregation, and cytokine cell receptor interactions. DEGs were also regulated by 250 miRNAs. In conclusion, we built a novel T + E-induced rat BPH model, and discovered potentially important genes, pathways, and miRNA-mRNA regulatory networks.
雌激素和雄激素之间的失衡可能是良性前列腺增生（BPH）的一个重要机制，但具体机制仍不清楚。我们使用由睾酮和雌二醇制成的混合缓释微丸（T + E）来刺激建立BPH大鼠模型。与仅使用雄激素的前列腺增生大鼠模型相比，新的前列腺增生大鼠模型在前列腺增生的宏观和病理特征方面表现更佳。我们使用RNA测序和生物信息学方法检测新型良性前列腺增生大鼠组与对照组前列腺组织之间的差异表达基因（DEG），共发现458个DEG，其中336个上调，122个下调。随后，逆转录定量聚合酶链反应（RT-qPCR）证实了测序结果的真实性。分析结果表明，驱动蛋白家族成员4a（Kif4a）和细胞增殖相关核抗原（Mki67）是蛋白质-蛋白质相互作用（PPI）网络中的核心基因。此外，我们发现这两个基因在多种癌组织、正常组织和癌细胞中彼此呈正相关。这些DEG主要参与有丝分裂核分裂、核染色体分离以及细胞因子细胞受体相互作用。DEG还受到250个微小RNA（miRNA）的调控。总之，我们建立了一种新型的T + E诱导的大鼠BPH模型，并发现了潜在的重要基因、信号通路以及miRNA-信使核糖核酸（mRNA）调控网络。
