Institute of Basic Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China.
Andrologia. 2020 Feb;52(1):e13386. doi: 10.1111/and.13386. Epub 2019 Nov 16.
The miRNAs are dysregulated in BPH. Rape bee pollen (RBP) is used to improve benign prostatic hyperplasia (BPH). Whether RBP treats BPH by regulating the dysregulated miRNAs remains unclear. Here, we identified miRNAs regulated along with the improvement of BPH by RBP in posterior lobes of prostate in rats. Firstly, to screened miRNAs might relate to improvement of BPH by RBP, we compared differentially expressed miRNAs between BPH model group and RBP group by high-throughput sequencing. As a result, 10 known miRNAs and 17 novel miRNA were up-regulated in RBP group, and 6 known and 13 novel miRNAs were down-regulated. Secondly, among the known miRNAs, we identified those that might relate to BPH by RT-qPCR, while only rno-miR-184 was screened, so we compared it among normal control group, BPH model group and RBP group. The results showed that rno-miR-184 was significantly lower expressed in BPH group, but up-regulated along with the improvement of BPH by RBP. Moreover, expression level of rno-miR-184 was no difference between RBP group and normal control level. Therefore, we considered that RBP might improve BPH through regulating expression of miRNAs like rno-miR-184 in prostate in rats.
miRNAs 在 BPH 中失调。油菜蜂花粉（RBP）用于改善良性前列腺增生（BPH）。RBP 是否通过调节失调的 miRNAs 来治疗 BPH 尚不清楚。在这里，我们鉴定了 RBP 改善前列腺大鼠后叶 BPH 时受调节的 miRNAs。首先，为了筛选可能与 RBP 改善 BPH 相关的 miRNAs，我们通过高通量测序比较了 BPH 模型组和 RBP 组之间差异表达的 miRNAs。结果显示，RBP 组中有 10 个已知 miRNA 和 17 个新 miRNA 上调，6 个已知 miRNA 和 13 个新 miRNA 下调。其次，在已知 miRNA 中，我们通过 RT-qPCR 鉴定了那些可能与 BPH 相关的 miRNA，结果只筛选出 rno-miR-184，因此我们在正常对照组、BPH 模型组和 RBP 组中比较了 rno-miR-184 的表达水平。结果表明，rno-miR-184 在 BPH 组中表达显著降低，但随着 RBP 改善 BPH 而上调。此外，rno-miR-184 的表达水平在 RBP 组和正常对照组之间没有差异。因此，我们认为 RBP 可能通过调节前列腺中 rno-miR-184 等 miRNA 的表达来改善 BPH。
