Ezati Masoumeh, Hashemi Amir, Zumberg Inna, Nasr Minoo Partovi, Fohlerova Zdenka
Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 3082/12, Brno, 61600, Czech Republic.
Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 3082/12, Brno, 61600, Czech Republic.
Macromol Biosci. 2025 May;25(5):e2400609. doi: 10.1002/mabi.202400609. Epub 2025 Jan 21.
Regenerating skin tissue remains a major challenge in medical science, especially due to the risk of scarring and prolonged healing, which becomes even more complicated in people with diabetes. Recent advancements have led to the creation of therapeutic dressings incorporating drug-delivery systems to tackle these issues. Exosomes (Exos) derived from mesenchymal stem cells (MSCs) have gained significant attention for mediating therapy without directly using cells, thanks to their natural anti-inflammatory and tissue repair properties mirroring those of MSCs. In this study, an advanced wound dressing combines chitosan (CS) and polyethylene glycol (PEG) hydrogel with adipose MSCs-derived Exos (ADMSCs-Exos). This composite, formed using a straightforward blending technique, is engineered to improve the healing process of severe skin injuries by steadily releasing Exos as the hydrogel degrades. The in vitro studies demonstrate that this hydrogel-exosome dressing greatly enhances endothelial cell migration, reduces oxidative stress, and promotes angiogenesis, crucial for effective wound healing. Additionally, real time-polymerase chain reaction (RT-PCR) analysis revealed significant upregulation of key genes involved in these processes, supporting the therapeutic potential of the hydrogel-Exo combination. These findings emphasize the potential of this hydrogel-Exos combination as an innovative and promising solution for advanced wound care.
再生皮肤组织仍然是医学科学中的一项重大挑战，尤其是由于存在疤痕形成风险和愈合时间延长的问题，而这在糖尿病患者中会变得更加复杂。最近的进展促使人们研发出了结合药物递送系统的治疗性敷料来解决这些问题。源自间充质干细胞（MSC）的外泌体（Exo）因其具有与MSC相似的天然抗炎和组织修复特性，能够在不直接使用细胞的情况下介导治疗，从而受到了广泛关注。在本研究中，一种先进的伤口敷料将壳聚糖（CS）和聚乙二醇（PEG）水凝胶与脂肪间充质干细胞衍生的外泌体（ADMSCs-Exo）相结合。这种复合材料采用简单的混合技术制成，旨在通过随着水凝胶降解稳定释放外泌体来改善严重皮肤损伤的愈合过程。体外研究表明，这种水凝胶-外泌体敷料极大地增强了内皮细胞迁移、降低了氧化应激并促进了血管生成，而这些对于有效的伤口愈合至关重要。此外，实时聚合酶链反应（RT-PCR）分析显示，参与这些过程的关键基因有显著上调，这支持了水凝胶-外泌体组合的治疗潜力。这些发现强调了这种水凝胶-外泌体组合作为一种创新且有前景的高级伤口护理解决方案的潜力。
