Pannequin Anaïs, Muselli Alain, Marcourt Laurence, Ferreira Queiroz Emerson, Quiros-Guerrero Luis-Manuel, Asakawa Yoshinori, Dounoue-Kubo Miwa, Wolfender Jean-Luc
Université de Corse, Unité Mixte de Recherche du Centre national de la recherche scientifique (UMR CNRS) SPE 6134, Laboratoire Chimie des Produits Naturels, Corte, France.
Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland.
Front Plant Sci. 2025 Jan 7;15:1470307. doi: 10.3389/fpls.2024.1470307. eCollection 2024.
Bryophytes are non-vascular plants that appeared on Earth before vascular plants. More than 24,000 species are reported worldwide, and only a small proportion have been studied. However, part of their biosynthetic potential has been unveiled and more than 1,600 terpenoids have been detected and identified. The study of bryophytes faces challenges due to their small size, and sociology, making it difficult to collect large amounts of uncontaminated samples. Additionally, their chemical specificity and the scarcity of chemical data specific to this branch further complicate their study. Traditionally, research on bryophytes has focused only on specific species or classes of compounds.
In contrast, our work proposes the first untargeted metabolite profiling investigation of a large collection of bryophytes (63 species) mainly issued from Corsican biodiversity. Metabolite profiling was performed by UHPLC-HRMS/MS and the data was extensively annotated using computational tools and molecular networking. This allowed us to describe in detail the chemical space covered by our collection and to establish comparisons between all the moss and liverwort species available. To validate some of the structural annotations, 3 liverworts () and 2 mosses ( and ), available in larger quantities were fractionated using high-resolution semi-preparative HPLC, yielding 20 pure compounds. Five of them were newly discovered.
This study highlights the main compositional differences between mosses and liverworts at the chemical class level. By analyzing given molecular network clusters, specific biosynthetic features or compounds that are characteristic of certain species are highlighted and discussed in detail.
苔藓植物是在维管植物之前出现在地球上的非维管植物。全世界报道的苔藓植物超过24000种，只有一小部分得到了研究。然而，它们的部分生物合成潜力已被揭示，已检测和鉴定出1600多种萜类化合物。由于苔藓植物体型小且具有社会学特征，收集大量未受污染的样本很困难，因此对苔藓植物的研究面临挑战。此外，它们的化学特异性以及该分支特定化学数据的稀缺性进一步使研究复杂化。传统上，对苔藓植物的研究仅集中在特定的物种或化合物类别上。
相比之下，我们的工作提出了对大量主要来自科西嘉岛生物多样性的苔藓植物（63种）进行首次非靶向代谢物谱分析研究。通过超高效液相色谱-高分辨质谱/质谱进行代谢物谱分析，并使用计算工具和分子网络对数据进行广泛注释。这使我们能够详细描述我们的样本所覆盖的化学空间，并对所有可用的苔藓和地钱物种进行比较。为了验证一些结构注释，对数量较多的3种地钱（ ）和2种苔藓（ 和 ）使用高分辨率半制备高效液相色谱进行分离，得到20种纯化合物。其中5种是新发现的。
本研究突出了苔藓和地钱在化学类别水平上的主要成分差异。通过分析给定的分子网络簇，突出并详细讨论了某些物种特有的特定生物合成特征或化合物。
