Basolo F, Fontanini G, Squartini F
Tumori. 1985 Feb 28;71(1):1-11. doi: 10.1177/030089168507100101.
Susceptible BALB/c virgin female mice were treated with 3-methylcholanthrene in almond oil (0.5%) by skin painting and killed at monthly intervals. The mammary glands were prepared as whole mounts for stereoscopic study of the glandular tree with removal of any focal change for histology. The results showed a marked disarrangement in the architecture of the glandular tree, a prevalent ductal tree, and an overcrowding of ducts. Focal hyperplastic changes were frequently observed, including hyperplastic alveolar nodules, hyperplastic ductal nodules, noduloids and intraductal epithelial growths, and their respective rates of malignant transformation observed at histology were 22%, 15%, 7% and 50%. Data suggest a different weight of these preneoplastic changes as sources of malignant transformation and a prevalent ductal origin of the methylcholanthrene-induced neoplasms.
将易感的BALB/c处女雌性小鼠用杏仁油（0.5%）中的3-甲基胆蒽进行皮肤涂抹处理，并每隔一个月处死一批。将乳腺制成整体标本，以便对腺树进行立体研究，并去除任何用于组织学检查的局灶性病变。结果显示腺树结构明显紊乱，导管树普遍存在，导管过度拥挤。经常观察到局灶性增生性改变，包括增生性肺泡结节、增生性导管结节、类结节和导管内上皮生长，组织学观察到它们各自的恶性转化率分别为22%、15%、7%和50%。数据表明这些癌前病变作为恶性转化来源的权重不同，且3-甲基胆蒽诱导的肿瘤普遍起源于导管。
