Sato Yusuke, Hatanaka Yusaku, Sato Yoshitaka, Matsumoto Kota, Osana Shion, Nagatomi Ryoichi, Nishizawa Seiichi
Department of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aramaki-Aza Aoba, Aoba-ku, Sendai 980-8578, Japan.
JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
Anal Chem. 2025 Mar 18;97(10):5480-5487. doi: 10.1021/acs.analchem.4c04852. Epub 2025 Jan 22.
Enveloped viruses have caused the majority of epidemics and pandemics over the past decade. Direct sensing of virus particles (virions) holds great potential for the functional analysis of enveloped viruses. Here, we explore a series of viral membrane-targeting amphipathic helical (AH) peptide-based molecular probes for the assessment of infectious titers of the human coronavirus 229E virus (HCoV-229E). The M2-protein-derived AH peptide is identified as a strong binder for HCoV-229E, and its conjugate with Nile Red, M2-NR, exhibits fluorogenic response upon selective binding to the viral membrane of HCoV-229E. We demonstrate that the response of M2-NR toward the HCoV-229E virus enables the rapid, simple, and reliable assessment of the infectivity of HCoV-229E. In addition, the present fluorescence assay for infectivity analysis is applicable to various kinds of enveloped virus including influenza A virus, herpes simplex virus-1, and lentivirus.
在过去十年中，包膜病毒引发了大多数的流行病和大流行。直接检测病毒颗粒（病毒体）对包膜病毒的功能分析具有巨大潜力。在此，我们探索了一系列基于病毒膜靶向两亲性螺旋（AH）肽的分子探针，用于评估人冠状病毒229E病毒（HCoV - 229E）的感染滴度。源自M2蛋白的AH肽被鉴定为HCoV - 229E的强结合剂，其与尼罗红的共轭物M2 - NR在与HCoV - 229E的病毒膜选择性结合时表现出荧光反应。我们证明M2 - NR对HCoV - 229E病毒的反应能够快速、简单且可靠地评估HCoV - 229E的感染性。此外，目前用于感染性分析的荧光测定法适用于包括甲型流感病毒、单纯疱疹病毒1型和慢病毒在内的各种包膜病毒。
