Nicholson Tracy L, Stuart Keira L, Bayles Darrell O
National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA, USA.
National Veterinary Services Laboratories, Animal Plant Health Inspection Service, USDA, Ames, IA, USA.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006631.
Three novel strains within the genus (29887, 29892 and 29896) were isolated from healthy pigs during routine veterinary physical exams. All three strains were non-motile and non-spore-forming Gram-positive cocci. The complete genome of each strain was attained, and phylogenetic analyses were performed. Comparison of the genomes of 29887, 29892 and 29896 to the genomes of other strains revealed digital DNA-DNA hybridization (dDDH) values between 21.2% and 53.9% and average nucleotide identity (ANI) values between 70.00% and 94.44%. Phylogenetic analyses suggested that each strain, 29896 ( sp. nov.), 29887 ( sp. nov.) and 29892 ( subsp. subsp. nov.), may represent a novel species within the genus , while ANI analysis indicated that strains 29896 ( sp. nov.) and 29887 ( sp. nov.) represent novel species within the genus and 29892 ( subsp. subsp. nov.) represents a novel subspecies of 29887 ( sp. nov.). Based upon the combined data presented in this study, two novel species, sp. nov. (type strain, 29896=NRRL B-65677=NCTC 14941) and sp. nov. (type strain, 29887=NRRL B-65675=NCTC 14939) are proposed, and one novel subspecies, subsp. subsp. nov. (type strain, 29892=NRRL B-65676=NCTC 14940) is proposed.
在常规兽医体检期间，从健康猪中分离出了该属内的三株新菌株（29887、29892和29896）。这三株菌株均为无运动性、不形成芽孢的革兰氏阳性球菌。获得了每株菌株的完整基因组，并进行了系统发育分析。将29887、29892和29896的基因组与其他该属菌株的基因组进行比较，结果显示数字DNA-DNA杂交（dDDH）值在21.2%至53.9%之间，平均核苷酸同一性（ANI）值在70.00%至94.44%之间。系统发育分析表明，每株菌株，即29896（新种）、29887（新种）和29892（亚种新亚种），可能代表该属内的一个新物种，而ANI分析表明，菌株29896（新种）和29887（新种）代表该属内的新物种，29892（亚种新亚种）代表29887（新种）的一个新亚种。基于本研究中呈现的综合数据，提出了两个新物种，即新种（模式菌株，29896 = NRRL B-65677 = NCTC 14941）和新种（模式菌株，29887 = NRRL B-65675 = NCTC 14939），并提出了一个新亚种，即亚种新亚种（模式菌株，29892 = NRRL B-65676 = NCTC 14940）。
