Carmo Raquel Alves do, Cörner Ana Carolina Organista, Martins Eduardo Ferreira, Ouverney Gabriel, Thurler Júnior Julio Cesar, Robbs Bruno Kaufmann, Pascoal Vinicius D'Avila Bitencourt, Esposito Elisa, Capelo Luciane Portas, Camargo Gabriela Alessandra da Cruz Galhardo
Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, Departamento de Clínica Odontológica, Nova Friburgo, Rio de Janeiro, Brasil.
Universidade Federal de São Paulo, Instituto de Ciência e Tecnologia da São José dos Campos, São Paulo, Brasil.
J Appl Oral Sci. 2025 Jan 20;33:e20240080. doi: 10.1590/1678-7757-2024-0080. eCollection 2025.
To evaluate the clinical effectiveness of ozonated sunflower oil (Oz) as an adjunctive of non-surgical periodontal therapy in patients with type 2 diabetes mellitus (DM2), on fibroblast cell viability and migration and the effectiveness of Oz on a Candida albicans (C. albicans) culture.
In total, 32 sites in 16 DM2 with moderate to advanced periodontal disease with periodontal pocket depths ≥5mm were selected. The treatments were divided into two groups: control, saline solution (SS) as an adjunctive of scaling and root planing (SRP+SS), and test, Oz as an adjunctive of SRP (SRP+Oz). Hematological [fasting glucose level (FGL) and hemoglobin A1c (HbA1c)] and microbiological samples were collected from the participants at baseline and three months after periodontal treatment and the microbiological samples were analyzed by PCR. C. albicans was previously tested by the agar diffusion test. The effect of Oz was tested on cell viability and fibroblast migration.
The groups showed no statistically significant differences (paired t-test-p>0.05) regarding hematological parameters, FGL (median - baseline 171.41, 3 months 164mg/dL), and HbA1c (baseline 8%, 3 months 7.5%) (Kruskal-Wallis One-Way Nonparametric-p>0.05) after periodontal therapy. The groups showed statistical differences for periodontal parameters between baseline and three months (paired t-test-p<0.05). PCR analysis showed a reduction in the percentage of C. albicans in the SRP+Oz group after three months (McNemar's test-p=0.002). Cell viability was lower in the high glucose Dulbecco's modified Eagle's medium (4500 mg/L) than in low glucose (1000 mg/L) (RM-ANOVA-p<0.0001). The wound healing test showed reduced fibroblast migration (one-way ANOVA with Dunnett's post-test-p<0.01). Oz showed high C. albicans antifungal inhibition (Kruskal-Wallis test-p=0.0001).
SRP+Oz effectively reduced C. albicans in-vitro and in-vivo but showed no clinical improvements compared to the control. Cell viability and wound healing of fibroblasts showed no improvements.
评估臭氧化葵花籽油（Oz）作为2型糖尿病（DM2）患者非手术牙周治疗辅助手段的临床效果，以及其对成纤维细胞活力和迁移的影响，还有Oz对白色念珠菌培养物的作用效果。
总共选取了16例患有中度至重度牙周病且牙周袋深度≥5mm的DM2患者的32个部位。治疗分为两组：对照组，用生理盐水（SS）作为龈下刮治和根面平整术（SRP + SS）的辅助手段；试验组，用Oz作为SRP的辅助手段（SRP + Oz）。在基线期以及牙周治疗后三个月从参与者处采集血液学样本[空腹血糖水平（FGL）和糖化血红蛋白（HbA1c）]以及微生物样本，并通过聚合酶链反应（PCR）分析微生物样本。白色念珠菌先前已通过琼脂扩散试验进行检测。检测Oz对细胞活力和成纤维细胞迁移的影响。
两组在牙周治疗后血液学参数方面无统计学显著差异（配对t检验 - p>0.05），FGL（中位数 - 基线期171.41，三个月后164mg/dL）以及HbA1c（基线期8%，三个月后7.5%）（Kruskal - Wallis单向非参数检验 - p>0.05）。两组在基线期和三个月后的牙周参数存在统计学差异（配对t检验 - p<0.05）。PCR分析显示三个月后SRP + Oz组白色念珠菌的百分比降低（McNemar检验 - p = 0.002）。在高糖（4500mg/L）的杜氏改良 Eagle培养基中细胞活力低于低糖（1000mg/L）培养基（重复测量方差分析 - p<0.0001）。伤口愈合试验显示成纤维细胞迁移减少（采用Dunnett事后检验的单向方差分析 - p<0.01）。Oz对白色念珠菌显示出高度抗真菌抑制作用（Kruskal - Wallis检验 - p = 0.0001）。
SRP + Oz在体外和体内均有效降低白色念珠菌，但与对照组相比未显示出临床改善。成纤维细胞的细胞活力和伤口愈合未显示出改善。
