Medić Ana, Milićević Tijana, Khraibah Abdullah, Herceg Romanić Snježana, Matek Sarić Marijana, Li Yingxi, D'Mello Rochelle, Berezovski Maxim, Popović Aleksandar, Minić Zoran, Karadžić Ivanka
University of Belgrade, Faculty of Medicine, Department of Chemistry, Višegradska 26, 11000 Belgrade, Serbia.
University of Belgrade, Institute of Physics Belgrade, a National Institute of the Republic of Serbia, Pregrevica 118, 11080 Belgrade, Serbia.
Food Chem. 2025 Apr 30;472:142959. doi: 10.1016/j.foodchem.2025.142959. Epub 2025 Jan 18.
This study integrates proteome analysis of human breast milk (HBM) from a homogeneous group of mothers who are of similar age and live in the same geographical area, along with an analysis of essential and potentially toxic elements in HBM in relation to lifestyle and environmental factors. This preliminary proteomic study, which examined 11 samples of HBM from lactating women, identified a total of 1619 proteins across all samples, revealing significant differences in proteomes influenced by lactation stages, parity, and exposure to tobacco smoke. The pilot study aimed to explore the feasibility of correlating certain proteins with several elements, considered as indicators of tobacco smoke and environmental influences on HBM. Notably, a clear and significant correlation was found between altered calcium content in HBM and the proteome fraction associated with calcium-binding proteins. The findings suggest that all analyzed factors impact the HBM proteome and the activity of certain enzymes.
本研究整合了来自年龄相仿且居住在同一地理区域的一组同质母亲的人乳（HBM）蛋白质组分析，以及对HBM中必需元素和潜在有毒元素与生活方式和环境因素关系的分析。这项初步蛋白质组学研究检测了11份哺乳期妇女的HBM样本，在所有样本中总共鉴定出1619种蛋白质，揭示了受哺乳阶段、胎次和接触烟草烟雾影响的蛋白质组存在显著差异。该试点研究旨在探索将某些蛋白质与几种元素相关联的可行性，这些元素被视为烟草烟雾和环境对HBM影响的指标。值得注意的是，在HBM中钙含量的改变与与钙结合蛋白相关的蛋白质组部分之间发现了明显且显著的相关性。研究结果表明，所有分析因素都会影响HBM蛋白质组和某些酶的活性。
