Salama A, Mueller-Eckhardt C, Bhakdi S
Vox Sang. 1985;48(4):239-45. doi: 10.1111/j.1423-0410.1985.tb00178.x.
A sensitive two-stage immunoradiometric assay (IRMA) using unlabeled specific antiglobulin sera followed by binding of radiolabeled staphylococcal protein A has been developed for detection of human IgG, IgM, IgA and complement C3 on human blood cells. In an experimental model system, purified human immunoglobulins (Ig) were coupled onto red blood cells, platelets, and leukocytes. The Ig-coated red blood cells were analyzed in parallel by three methods: the radioimmune antiglobulin test with radiolabeled anti-Ig; the radiolabeled staphyloccal protein A test, and the IRMA. Of the three methods evaluated, the latter was found to be sensitive and the easiest to perform. The applicability of the IRMA was established by investigating a group of 14 selected patients with autoimmune warm type hemolytic anemia. We observed that the IRMA detected cell-bound antibodies (IgG and/or IgM) in several cases where conventional assays yielded negative results.
一种灵敏的两步免疫放射分析（IRMA）方法已被开发出来，用于检测人血细胞上的人IgG、IgM、IgA和补体C3。该方法先使用未标记的特异性抗球蛋白血清，然后结合放射性标记的葡萄球菌蛋白A。在一个实验模型系统中，将纯化的人免疫球蛋白（Ig）偶联到红细胞、血小板和白细胞上。对Ig包被的红细胞通过三种方法进行平行分析：用放射性标记的抗Ig进行放射免疫抗球蛋白试验；放射性标记的葡萄球菌蛋白A试验，以及IRMA。在所评估的三种方法中，发现后者灵敏且最易于操作。通过对一组14例选定的自身免疫性温抗体型溶血性贫血患者进行研究，确立了IRMA的适用性。我们观察到，在一些常规检测结果为阴性的病例中，IRMA检测到了细胞结合抗体（IgG和/或IgM）。
