Loomis K, Kickler T S, Sears D, Ness P M, Perlstein M T, Johnson R
Am J Clin Pathol. 1985 Jan;83(1):12-7. doi: 10.1093/ajcp/83.1.12.
A two-step, solid-phase radioimmunoassay for the measurement of antibodies directed against platelets, neutrophils, and red blood cells is described. Cells to be tested or standard solutions of human IgG (HIgG) first are incubated with excess 125I goat anti-human IgG. Unbound 125I anti-IgG is adsorbed in a second step to polystyrene balls (PB) coated with excess human IgG. Inhibition of binding in this second step is related directly to the level of cell associated IgG. The amount of cell bound IgG is determined by comparison to a inhibition curve for standard IgG solution. The method was evaluated by quantitating antibody levels in patients with clinical evidence of immune cytopenias.
本文描述了一种用于检测抗血小板、中性粒细胞和红细胞抗体的两步固相放射免疫测定法。首先将待测细胞或人IgG（HIgG）标准溶液与过量的125I山羊抗人IgG孵育。在第二步中，未结合的125I抗IgG被吸附到包被有过量人IgG的聚苯乙烯球（PB）上。第二步中结合的抑制与细胞相关IgG的水平直接相关。通过与标准IgG溶液的抑制曲线比较来确定细胞结合IgG的量。通过对有免疫性血细胞减少症临床证据的患者的抗体水平进行定量来评估该方法。
