High-throughput screening strategies for plastic-depolymerizing enzymes.

A multitude of plastic-depolymerizing microorganisms and enzymes have been discovered in the plastisphere. Identifying and engineering such microbial strains and enzymes necessitate robust and high-throughput screening strategies for developing effective microbial solutions to counter the plastic accumulation problem and decouple the reliance on fossil resources. This review covers new methods and approaches for the effective high-throughput screening of depolymerizing enzymes for various plastics, such as polyethylene terephthalate (PET), polyurethane (PU), and polylactic acid (PLA). We discuss the application scope of the existing methods, as well as potential developments and integration of screening techniques to identify and enhance plastic depolymerases. The prospects for screening a wider range of plastic depolymerases with the advances in biotechnology tools such as droplet microfluidics and biosensors are highlighted.