Department of Horticulture, Abhar Branch, Islamic Azad University, Abhar, Iran.
Department of Horticultural Science, Imam Khomeini International University, Qazvin, 34148- 96818, Iran.
Sci Rep. 2024 Oct 28;14(1):25802. doi: 10.1038/s41598-024-77358-x.
This study investigated postharvest dopamine treatment efficiency in ameliorating chilling injury of banana fruits during storage at 7 ºC for 21 days. Our results showed that dopamine treatment at 150 µM promoted phenols and flavonoids biosynthesis acquired by higher phenylalanine ammonia-lyase (PAL) expression and activity concurrent with lower polyphenol oxidase (PPO) expression and activity leading to higher DPPH, FRAP, and ABTS radicals scavenging activity. In addition, dopamine treatment at 150 µM promoted endogenous proline biosynthesis by activating pyrroline-5-carboxylate synthetase (P5CS) and ornithine δ-aminotransferase (OAT) expression and activity concurrent with suppressing proline dehydrogenase (ProDH) expression and activity. Furthermore, higher endogenous γ-aminobutyric acid (GABA) biosynthesis in banana fruits by 150 µM dopamine treatment was accompanied by higher glutamate decarboxylase (GAD) and GABA transaminase (GABA-T) expression and activity. Therefore, our results suggest that dopamine treatment at 150 µM might be employed for banana fruits chilling injury amelioration by enhancing phenylpropanoid pathway activity and boosting endogenous proline and GABA biosynthesis.
本研究调查了采后多巴胺处理在改善香蕉果实冷藏损伤方面的效率，贮藏温度为 7℃，时间为 21 天。我们的结果表明，150µM 的多巴胺处理通过提高苯丙氨酸氨裂解酶（PAL）的表达和活性，同时降低多酚氧化酶（PPO）的表达和活性，促进了酚类和类黄酮的生物合成，从而获得更高的 DPPH、FRAP 和 ABTS 自由基清除活性。此外，150µM 的多巴胺处理通过激活吡咯啉-5-羧酸合成酶（P5CS）和鸟氨酸 δ-氨基转移酶（OAT）的表达和活性，同时抑制脯氨酸脱氢酶（ProDH）的表达和活性，促进了内源脯氨酸的生物合成。此外，150µM 多巴胺处理的香蕉果实中，内源性γ-氨基丁酸（GABA）的生物合成较高，伴随着谷氨酸脱羧酶（GAD）和 GABA 转氨酶（GABA-T）的表达和活性较高。因此，我们的结果表明，150µM 的多巴胺处理可能通过增强苯丙烷途径的活性和促进内源脯氨酸和 GABA 的生物合成来改善香蕉果实的冷害。
