Marques Irene F, Domènech-Panicello Carola, Geurtsen Madelon L, Hoang Thanh T, Richmond Rebecca, Polinski Kristen, Sirignano Lea, Page Christian M, Binter Anne-Claire, Everson Todd, Burt Amber, Deuschle Michael, Gilles Maria, Streit Fabian, Mumford Sunni L, Magnus Per, Reiss Irwin K M, Vermeulen Marijn J, Witt Stephanie H, Chaves Inês, Yeung Edwina, London Stephanie J, Guxens Mònica, Felix Janine F
Generation R Study Group, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
Department of Pediatrics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.
Clin Epigenetics. 2025 Jan 22;17(1):12. doi: 10.1186/s13148-024-01810-y.
Night shift work during pregnancy has been associated with differential DNA methylation in placental tissue, but no studies have explored this association in cord blood. We aimed to examine associations of maternal night shift work with cord blood DNA methylation.
A total of 4487 mother-newborn pairs from 7 studies were included. Maternal night shift work during pregnancy was ascertained via questionnaires and harmonized into "any" versus "no". DNA methylation was measured in cord blood using the Illumina Infinium Methylation arrays. Robust linear regression models adjusted for relevant confounders were run in the individual cohorts, and results were meta-analyzed.
Maternal night shift work during pregnancy ranged from 3.4% to 26.3%. Three CpGs were differentially methylated in relation to maternal night shift work during pregnancy at a false discovery rate adjusted P < 0.05: cg10945885 (estimate (β) 0.38%, standard error (SE) 0.07), cg00773359 (β 0.25%, SE 0.05), and cg21836426 (β - 0.29%, SE 0.05). Associations of the identified CpGs were found in previous literature for gestational age and childhood and adolescent BMI. In a mouse model of prenatal jet lag exposure, information on offspring DNA methylation of ten homologous genes annotated to the 16 CpGs with P < 1 × 10 in our analysis was available, of which eight were associated (enrichment P: 1.62 × 10).
Maternal night shift work during pregnancy was associated with newborn DNA methylation at 3 CpGs. Top findings overlapped with those in a mouse model of gestational jet lag. This work strengthens evidence that DNA methylation could be a marker or mediator of impacts of circadian rhythm disturbances.
孕期夜班工作与胎盘组织中DNA甲基化差异有关，但尚无研究探讨其与脐带血中这种关联。我们旨在研究孕妇夜班工作与脐带血DNA甲基化之间的关联。
纳入了来自7项研究的4487对母婴。通过问卷调查确定孕期母亲的夜班工作情况，并统一分为“有”和“无”。使用Illumina Infinium甲基化芯片测量脐带血中的DNA甲基化。在各个队列中运行针对相关混杂因素进行调整的稳健线性回归模型，并对结果进行荟萃分析。
孕期母亲夜班工作的比例在3.4%至26.3%之间。在错误发现率调整后的P<0.05水平上，有3个CpG位点的甲基化与孕期母亲夜班工作存在差异：cg10945885（估计值（β）0.38%，标准误（SE）0.07）、cg00773359（β 0.25%，SE 0.05）和cg21836426（β -0.29%，SE 0.05）。在先前的文献中发现，所确定的这些CpG位点与胎龄以及儿童和青少年的体重指数有关。在产前时差暴露的小鼠模型中，可获得我们分析中16个P<1×10的CpG位点注释的10个同源基因的后代DNA甲基化信息，其中8个存在关联（富集P：1.62×10）。
孕期母亲夜班工作与新生儿3个CpG位点的DNA甲基化有关。主要发现与孕期时差反应小鼠模型中的结果重叠。这项工作强化了DNA甲基化可能是昼夜节律紊乱影响的标志物或介导因素的证据。
