Yemata Getahun, Yihune Endalamaw, Kebede Yewulsew
Department of Biology, College of Science, Bahir Dar University, P. O. Box 79, Bahir Dar, Ethiopia.
ScientificWorldJournal. 2025 Jan 15;2025:9481587. doi: 10.1155/tswj/9481587. eCollection 2025.
The present study was aimed to verify the medicinal value of and traditionally used to treat human and animal ailments in Ethiopia. Fresh leaves of these species were collected, dried under shade, and ground into fine powder. The extraction was carried out by the maceration method using methanol as a solvent. The compounds in the crude extract were further profiled by thin layer chromatography (TLC). The antibacterial activity of the compounds was evaluated using the agar well diffusion method. . had a higher percentage extract yield (22.5%) than . (13.7%). The phytochemical screening showed more abundant phenolic compounds and tannins in the extract of . and alkaloid, flavonoid, phenolic compounds, and tannins in the extract of . . Overall, . produced twofold higher growth inhibition zone (24.0 ± 0.5-43.0 ± 1.0 mm) against the tested pathogens than . (7.0 ± 0.5-24.0 ± 0.3 mm). Among the bacteria, was found to be the most sensitive to compounds of . . The lowest minimum inhibitory concentration (MIC) values (25 mg/mL) were obtained from compounds 1 and 3 of . leaf extract against and . and compounds 2 and 3 of the same against . . Similarly, compound 1 of . leaf extract produced the lowest MIC (25 mg/mL) against . and and compound 3 of the same species against . and . . All the profiled compounds of the two species had both bactericidal and bacteriostatic properties against the tested pathogens. The results of the present study revealed that the compounds of . had strong antibacterial activity against all the tested pathogens, indicating the high potential of the compounds. However, further characterization and identification of the specific compounds for the development of biocide formulations are suggested.
本研究旨在验证埃塞俄比亚传统上用于治疗人类和动物疾病的[物种名称1]和[物种名称2]的药用价值。采集了这些物种的新鲜叶子，在阴凉处干燥并研磨成细粉。采用浸渍法，以甲醇为溶剂进行提取。粗提物中的化合物通过薄层色谱（TLC）进一步分析。使用琼脂孔扩散法评估化合物的抗菌活性。[物种名称1]的提取物产率（22.5%）高于[物种名称2]（13.7%）。植物化学筛选表明，[物种名称1]提取物中的酚类化合物和单宁含量更为丰富，[物种名称2]提取物中含有生物碱、黄酮类化合物、酚类化合物和单宁。总体而言，[物种名称1]对受试病原体产生的生长抑制圈（24.0±0.5 - 43.0±1.0毫米）是[物种名称2]（7.0±0.5 - 24.0±0.3毫米）的两倍。在细菌中，[具体细菌名称]被发现对[物种名称1]的化合物最敏感。[物种名称1]叶提取物的化合物1和3对[具体细菌名称1]和[具体细菌名称2]以及相同提取物的化合物2和3对[具体细菌名称3]获得了最低最低抑菌浓度（MIC）值（25毫克/毫升）。同样，[物种名称2]叶提取物的化合物1对[具体细菌名称4]和[具体细菌名称5]产生了最低MIC（25毫克/毫升），该物种的化合物3对[具体细菌名称6]和[具体细菌名称7]产生了最低MIC。这两个物种的所有分析化合物对受试病原体均具有杀菌和抑菌特性。本研究结果表明，[物种名称1]的化合物对所有受试病原体具有强大的抗菌活性，表明这些化合物具有很高的潜力。然而，建议进一步表征和鉴定用于开发杀生物剂配方的特定化合物。
