Study on Antibacterial Activities of and Leaf Extracts Against Some Human Pathogens.

The present study was aimed to verify the medicinal value of and traditionally used to treat human and animal ailments in Ethiopia. Fresh leaves of these species were collected, dried under shade, and ground into fine powder. The extraction was carried out by the maceration method using methanol as a solvent. The compounds in the crude extract were further profiled by thin layer chromatography (TLC). The antibacterial activity of the compounds was evaluated using the agar well diffusion method. . had a higher percentage extract yield (22.5%) than . (13.7%). The phytochemical screening showed more abundant phenolic compounds and tannins in the extract of . and alkaloid, flavonoid, phenolic compounds, and tannins in the extract of . . Overall, . produced twofold higher growth inhibition zone (24.0 ± 0.5-43.0 ± 1.0 mm) against the tested pathogens than . (7.0 ± 0.5-24.0 ± 0.3 mm). Among the bacteria, was found to be the most sensitive to compounds of . . The lowest minimum inhibitory concentration (MIC) values (25 mg/mL) were obtained from compounds 1 and 3 of . leaf extract against and . and compounds 2 and 3 of the same against . . Similarly, compound 1 of . leaf extract produced the lowest MIC (25 mg/mL) against . and and compound 3 of the same species against . and . . All the profiled compounds of the two species had both bactericidal and bacteriostatic properties against the tested pathogens. The results of the present study revealed that the compounds of . had strong antibacterial activity against all the tested pathogens, indicating the high potential of the compounds. However, further characterization and identification of the specific compounds for the development of biocide formulations are suggested.