Macedo-Mendoza M, Calderón-Vallejo D, González-Torres M L, Martínez-Martínez A, Gasca-Martínez D, Orta Salazar E M, Quintanar J L
Universidad Autónoma de Aguascalientes, Centro de Ciencias Básicas, Department of Physiology and Pharmacology.
Department of Morphology.
Acta Endocrinol (Buchar). 2024 Apr-Jun;20(2):127-135. doi: 10.4183/aeb.2024.127. Epub 2025 Jan 18.
Studies indicate a decrease in spatial memory across species as they age. Moreover, consistent administration of Gonadotropin-releasing hormone (GnRH) improves learning abilities in older rats that have undergone gonadectomy.
The aim of this study was to investigate the effects of the GnRH agonist, leuprolide acetate (LA) on spatial memory in aged intact male rats and the expression of proteins associated with hippocampal plasticity.
Aged male rats were injected with LA or saline every three days for nine weeks to assess the effects of LA on spatial memory. The aged rats were trained in the Morris Water Maze for four days, and the evaluation took place on the fifth day. Additionally, the Y-maze test was used to investigate short-term spatial memory. The expression of spinophilin and microtubule-associated protein 2 (MAP2) in the hippocampus was measured.
Behavioral tests revealed that LA improves spatial memory in aged rats. Additionally, we observed increased expression of spinophilin and MAP2 in the hippocampus of aged male rats following LA treatment. Based on these results, the administration of LA holds is a potential treatment for restoring cognitive function in aged individuals.
研究表明，随着年龄增长，各物种的空间记忆能力都会下降。此外，持续给予促性腺激素释放激素（GnRH）可提高已接受去势手术的老年大鼠的学习能力。
本研究旨在探讨GnRH激动剂醋酸亮丙瑞林（LA）对老龄未阉割雄性大鼠空间记忆及海马可塑性相关蛋白表达的影响。
对老龄雄性大鼠每三天注射一次LA或生理盐水，持续九周，以评估LA对空间记忆的影响。对老龄大鼠进行四天的莫里斯水迷宫训练，并在第五天进行评估。此外，采用Y迷宫试验研究短期空间记忆。检测海马中亲嗜素和微管相关蛋白2（MAP2）的表达。
行为测试表明，LA可改善老龄大鼠的空间记忆。此外，我们观察到LA处理后老龄雄性大鼠海马中亲嗜素和MAP2的表达增加。基于这些结果，LA给药有望成为恢复老年人认知功能的一种治疗方法。
