Liao Tzu-Ying, Boden Andrew, King Peter C, Thissen Helmut, Crawford Russell J, Ivanova Elena P, Kingshott Peter
Department of Chemistry and Biotechnology; School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
ACS Appl Bio Mater. 2025 Feb 17;8(2):1167-1185. doi: 10.1021/acsabm.4c01380. Epub 2025 Jan 23.
Despite their widespread utilization in biomedical applications, these synthetic materials can be susceptible to microbial contamination, potentially compromising their functionality and increasing the risk of infection in patients. In this study, molybdenum (Mo), an essential metal in biological systems, was investigated as a Mo-based cold-sprayed coating on poly(dimethylsiloxane) (PDMS) for its potential use as biocompatible and antimicrobial surfaces for biomedical applications. Various cold-spray parameters were employed in the fabrication of Mo-embedded PDMS surfaces to alter the surface structure of the substrate, Mo loading density, and embedding layer thickness. Specifically, relatively low nozzle scanning speeds were used to develop high-density Mo-embedded PDMS surfaces. A comprehensive analysis was conducted to investigate how cold-spray processing parameters affect the surface topography, wettability, and chemical properties. The ability of the Mo-embedded PDMS to inhibit the colonization of , , , and bacterial species was demonstrated by both live/dead staining and disk diffusion methods. Surfaces with higher Mo loading densities significantly reduced the level of bacterial attachment and enhanced the bactericidal activity upon contact. Also, the level of Mo ion release over a 14-day period was measured and correlated to the properties of the substrate surface. Furthermore, attachment, viability, and proliferation of osteoblast-like MG63 cells were assessed to investigate the effect of Mo ion release on the biocompatibility of fabricated coatings. A notable decrease in cell viability and delayed growth of MG63 cells became evident after 7 days of incubation with the highly Mo-loaded samples. While this study enhanced our understanding regarding the engineering of composite materials for combatting microbial infections, the findings also suggest that the release of Mo ions may detrimentally affect osteoblast survival, potentially compromising the long-term functionality of orthopedic implants produced using this technique.
尽管这些合成材料在生物医学应用中被广泛使用，但它们可能容易受到微生物污染，这可能会损害其功能并增加患者感染的风险。在本研究中，钼（Mo）作为生物系统中的一种必需金属，被研究用于在聚二甲基硅氧烷（PDMS）上制备基于钼的冷喷涂涂层，以用作生物医学应用中的生物相容性和抗菌表面。在制备嵌入钼的PDMS表面时采用了各种冷喷涂参数，以改变基底的表面结构、钼负载密度和嵌入层厚度。具体而言，使用相对较低的喷嘴扫描速度来制备高密度嵌入钼的PDMS表面。进行了全面分析，以研究冷喷涂工艺参数如何影响表面形貌、润湿性和化学性质。通过活/死染色和纸片扩散法证明了嵌入钼的PDMS抑制金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌细菌物种定殖的能力。具有较高钼负载密度的表面显著降低了细菌附着水平，并增强了接触时的杀菌活性。此外，还测量了14天内钼离子的释放水平，并将其与基底表面的性质相关联。此外，评估了成骨样MG63细胞的附着、活力和增殖，以研究钼离子释放对制备涂层生物相容性的影响。与高钼负载样品孵育7天后，MG63细胞的活力明显下降，生长延迟。虽然这项研究增进了我们对用于对抗微生物感染的复合材料工程的理解，但研究结果也表明，钼离子的释放可能会对成骨细胞存活产生不利影响，从而可能损害使用该技术生产的骨科植入物的长期功能。
