Weems W A, Seidel E R, Johnson L R
Am J Physiol. 1985 Apr;248(4 Pt 1):G470-8. doi: 10.1152/ajpgi.1985.248.4.G470.
Experiments were conducted in vitro to determine whether arterial infusion of the C-terminal octapeptide of cholecystokinin (CCK-8) into cat jejunal segments could elicit propulsive behavior when a segment must do hydrostatic work to expel fluid. Oral and aboral ends of jejunal segments, 17 cm in length, were connected to a propulsion evaluation system that imposed input-output conditions of constant capacitance and negligible resistance. Infusion of 1 X 10(-10) to 3 X 10(-9) M CCK-8 produced an initial simultaneous ejection of approximately equal volumes of fluid from both ends of the segments. This reduction in luminal volume lasted for approximately 4 min. Concurrent with this initial reduction in luminal volume were phasic ejections of fluid that often did not occur simultaneously or with equal magnitude from both ends of a segment. This pattern persisted throughout the period of CCK-8 infusion. Arterial infusion of atropine sulfate (10(-6) M) or hexamethonium sulfate (2.8 X 10(-7) M) prevented the induction of propulsive behavior by CCK-8. These results indicate that arterial infusion of CCK-8 can induce a specific pattern of jejunal propulsive behavior by triggering neural activity in the enteric nervous system and that CCK-8 may have a physiological role in regulating propulsion.
进行了体外实验，以确定在猫空肠段必须做静水功以排出液体时，向其动脉内注入胆囊收缩素C末端八肽（CCK - 8）是否能引发推进行为。将长度为17厘米的空肠段的口腔端和肛门端连接到一个推进评估系统，该系统施加恒定电容和可忽略电阻的输入 - 输出条件。注入1×10⁻¹⁰至3×10⁻⁹ M的CCK - 8会使空肠段两端同时初步排出大致等量的液体。管腔容积的这种减少持续约4分钟。与管腔容积的这种初步减少同时发生的是液体的阶段性排出，同一空肠段两端的这种排出通常不同时发生，且量也不相等。在整个CCK - 8注入期间，这种模式一直持续。动脉内注入硫酸阿托品（10⁻⁶ M）或硫酸六甲铵（2.8×10⁻⁷ M）可阻止CCK - 8诱导推进行为。这些结果表明，动脉内注入CCK - 8可通过触发肠神经系统中的神经活动来诱导空肠推进行为的特定模式，并且CCK - 8可能在调节推进方面具有生理作用。
