Alizadeh H, Weems W A, Castro G A
Department of Physiology and Cell Biology, University of Texas Medical School, Houston.
Gastroenterology. 1989 Dec;97(6):1461-8. doi: 10.1016/0016-5085(89)90390-9.
The intrinsic fluid-propelling behavior of guinea pig jejunal segments was reportedly altered by primary infection with Trichinella spiralis from day 10 to day 20 postinoculation. After that time propulsive behavior returned to normal. The objective in the investigation reported here was to determine if jejunal responsiveness to an infection is influenced by prior exposure to the parasite. Accordingly, jejunal propulsion was examined in guinea pigs reinfected 2 mo after the initial inoculation with the parasite. Propulsive behavior was measured in vitro by attaching oral and aboral ends of jejunal segments to a propulsion evaluation system that imposed input-output conditions of constant capacitance and negligible resistance. Propulsive complexes produced by segments removed from animals 60 days after primary inoculation were generally similar in configuration to those produced by segments from uninfected hosts. The pronounced net aboral propulsive behavior previously observed 10-20 days after primary infection was not observed in segments from animals 60 days postinfection or from uninfected controls. After secondary inoculation the fluid volume expelled per propulsive complex began to increase within 1 day and net aboral propulsive behavior was clearly evident 2 days postinoculation. As net aboral propulsive behavior was induced approximately five times faster after secondary as compared with primary inoculation with T. spiralis, it is evident that the overall intrinsic mechanisms that are responsible for an earlier onset of net aboral propulsive behavior are responsive for at least 2 mo after initial exposure to the parasite.
据报道，从接种旋毛虫后的第10天到第20天，豚鼠空肠段的内在液体推进行为因初次感染而发生改变。在那之后，推进行为恢复正常。此处报道的研究目的是确定空肠对感染的反应性是否受先前接触该寄生虫的影响。因此，在初次接种寄生虫2个月后再次感染的豚鼠中检查空肠推进情况。通过将空肠段的口端和肛端连接到一个施加恒电容和可忽略电阻的输入-输出条件的推进评估系统上，在体外测量推进行为。初次接种60天后从动物身上取出的肠段产生的推进复合体在形态上通常与未感染宿主的肠段产生的推进复合体相似。在初次感染后10 - 20天观察到的明显的净肛向推进行为在感染后60天的动物肠段或未感染对照的肠段中未观察到。二次接种后，每个推进复合体排出的液体量在1天内开始增加，接种后2天净肛向推进行为明显。由于与初次接种旋毛虫相比，二次接种后净肛向推进行为的诱导速度快约五倍，显然，负责净肛向推进行为更早出现的整体内在机制在初次接触寄生虫后至少2个月内仍有反应。
