Hidalgo-Vicelis José Luis, Rivera-Contreras Angélica Raquel, Hernández-Téllez Beatriz, Piñón-Zárate Gabriela, Jarquín-Yáñez Katia, Fiordelisio-Coll Tatiana, Saniger-Blesa José Manuel, González-Gómez Gertrudis Hortensia, Falcón-Neri María Alicia, Canales-Martínez María Margarita, Castell-Rodríguez Andrés Eliú
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, Mexico.
Laboratory of Comparative Neuroendocrinology, Department of Biology, Faculty of Sciences, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, Mexico.
Gels. 2025 Jan 9;11(1):53. doi: 10.3390/gels11010053.
Currently, there are no therapies that prevent the negative myocardial remodeling process that occurs after a heart attack. Injectable hydrogels are a treatment option because they may replace the damaged extracellular matrix and, in addition, can be administered minimally invasively. Reactive oxygen species generated by ischemia-reperfusion damage can limit the therapeutic efficacy of injectable hydrogels. In order to overcome this limitation, grape seed proanthocyanidins were incorporated as antioxidant compounds into a thermosensitive myocardial extracellular matrix hydrogel in this study. For the fabrication of the hydrogel, the extracellular matrix obtained by decellularization of porcine myocardium was solubilized through enzymatic digestion, and the proanthocyanidins were incorporated. After exposing this extracellular matrix solution to 37 °C, it self-assembled into a hydrogel with a porous structure. According to the physicochemical and biological evaluation, the coupling of proanthocyanidins in the hydrogel has a positive effect on the antioxidant capacity, gelation kinetics, in vitro degradation, and cardiomyocyte viability, indicating that the hydrogel coupled with this type of antioxidants represents a promising alternative for potential application in post-infarction myocardial regeneration. Furthermore, this study proposes the best concentrations of proanthocyanidins that resulted in the hydrogels for future studies in cardiac tissue engineering.
目前，尚无疗法能够阻止心脏病发作后发生的负面心肌重塑过程。可注射水凝胶是一种治疗选择，因为它们可以替代受损的细胞外基质，此外，还可以通过微创方式给药。缺血再灌注损伤产生的活性氧会限制可注射水凝胶的治疗效果。为了克服这一限制，在本研究中，将葡萄籽原花青素作为抗氧化化合物掺入热敏心肌细胞外基质水凝胶中。为了制备水凝胶，通过酶消化使通过猪心肌脱细胞获得的细胞外基质溶解，并掺入原花青素。将这种细胞外基质溶液暴露于37°C后，它会自组装成具有多孔结构的水凝胶。根据物理化学和生物学评估，水凝胶中原花青素的偶联对抗氧化能力、凝胶化动力学、体外降解和心肌细胞活力具有积极影响，表明与这种类型抗氧化剂偶联的水凝胶是梗死心肌再生潜在应用的有前景的替代方案。此外，本研究提出了导致水凝胶产生的原花青素的最佳浓度，以供未来心脏组织工程研究使用。
